Figures and data
Major T-cell infiltration patterns observed in solid tumors.
A: Lack of tumor antigen, inadequate priming, defects in antigen presentation and/or lack of presentation, and/or lack of T-cell-attracting chemokines result in the absence of T-cells in the tumor. B: Presence of T-cells in invasive margins but absent in the tumor bed. Immune evasion may be due to stromal barriers, lack of chemokines, aberrant vasculature, or hypoxia. C: High degree of T-cell infiltration forms a hot tumor.
Structural organization in human NSCLC.
Staining was performed by multiplex IHC. FFPE NSCLC sections were stained for keratin as a marker of cancer cells (gray), CD3 (yellow), and fibroblast markers αSMA (green), FAP (red). First row: CD3 excluded patient. (a): scheme of the section showing CD3+ cell exclusion from tumor nests. The green arrow highlights border regions with contractile fibroblast barrier αSMA+FAP+ and low CD3+ cells. (b): CD3+ cells are localized in the center of the stroma. (c): dense αSMA staining at the tumor border are associated with a decrease of CD3+ cell abundance. (d): recap of all the markers. 2nd row: CD3 infiltrated patient. (e): scheme f the section showing CD3+ infiltration in the tumor islets. The green arrow shows αSMA+ staining on vessels. (f): CD3+ are localized in the tumor nest. (g): FAP+ staining is localized throughout the stroma. (h): recap of all the markers.
Composition of the tumor microenvironment.
This table presents the literature about the fraction of different species in the lung TME. The values found in the present studies are written in the two last rows. Different methods have been used, in different subtypes of lung cancer. We introduce the following abbreviations. (C-c): Cancer cells. (MΦ): Macrophages. (T-c): T-cells. (Fb): Fibroblasts. (scRNA-seq): single-cell RNA seq,(NSCLC): Non Small Cell Lung Cancer, (LUAD): Adenocarcinoma, (SSN): Sub-Solid Nodule. (S): stroma region. (T): tumor nest region. (ST): Stroma+tumor nest region. (TSR): tumor stroma ratio. Data for Ref. [31, 32, 33, 34, 35] were extracted from [38].
Scaling variation and estimation of the coefficients according to different scenarios.
The scenarios are described in Section II.2 and shown in Fig. 3. The coefficients above are those introduced in Eqs. (1)-(4). The left column summarizes the different roles that T-cells can play in a cell mixture and the values of the coefficients of the mixture are listed in the following horizontal line of the table. The scaling of δ is always 1, and αNA ∼ δϵ4.
Evolution of the TME composition over time.
Different profiles are obtained from Eqs. (1)-(4) according to the set of parameters reported in Table. 2 and consistent with the scenarios shown in Fig. 1. In all the plots, δ = 1 and αNA = 10−4. a,b,c,d: Immune-desert tumor. Cancer cells proliferate when the immune response is inefficient or when T-cells are not attracted to the tumor. e: Immune-excluded tumor. The CAF barrier inhibits T-cell infiltration, and promotes tumor growth. f,g,h: Immune-inflamed tumor. T-cell infiltration limits cancer cell growth. i: Density plot of the equilibrium cancer cell fraction at fixed δ and αNA, in function of the two parameters that control the growth: αTCδCT /(αNA,CKδTF) reflects the ability of T-cells to kill the tumor, and αNA,C + αTC reflects the competition between species for space and resources.
Values of the parameters in the spatial model
Small solid tumor growth.
We refer here to the different tumor phenotypes described in Fig. 1. A: Mass fraction of cancer cells at time t = 0 and profile of different mass fractions on a section of the tumor. B-C-D: Mass fraction of cancer cells at time t = 35T0 and profile of mass fraction in immunodeficient tumor (B), when immune infiltration is inhibited by CAFs (C), and in immune-inflamed tumor (D). E-F-G: Development of different zones. E: Surface fraction profile of the tumor nest for different scenarios, calculated as S/Stotal = ∫ drδ(C > 0.1)/Stotal. F: Fibrotic surface fraction profile for different scenarios, calculated as S/Stotal = ∫ drδ(T > 0.1)/Stotal. G: T-cell rich area fraction profile for different scenarios, calculated as S = ∫ drδ (T > 0.1)/Stotal. H: Cancer cell average mass fractions. I: CAF average mass fractions. J: T-cell average mass fractions. K: NAF average mass fractions.
Growth process of two small solid tumors.
Density plots showing cancer cell population. We start with two tumor nests placed at the same distance from the center of the domain (30µm), sharing the same mass fraction of cancer cells and we present different scenarios. A: Nests in immune-inflamed tumor. B: Nests interacting in immune-excluded tumor, with chemotactic T-cells and CAFs. C: Nests coalesce in immune-desert tumor, i.e. in the presence of inefficient T-cells.
Free growth of a tumor nest.
A-G: Cancer population density plots for different scenarios: Cancer cells (C-c)+NAFs+Inefficient T-cells, Cancer cells+NAFs,Cancer cells alone. A: Mass fraction of cancer cells at time t = 0 and profile of different mass fractions on a section of the tumor. B: Mass fraction of cancer cells at time t = 35T0 and mass fraction profile for a case of a stroma composed by C-c, NAFs, and inefficient T-cells. C: Mass fraction of cancer cells at time t = 35T0 and profile of the different mass fractions on a cut of the tumor for a case of a stroma composed by C-c and NAFs. D: Mass fraction of cancer cells at time t = 35T0 and mass fraction profile for a case with no stroma in the tumor microenvironment. E: Surface fraction of the tumor nest in the different scenarios. F: Average mass fraction of the cancer cells in the different scenarios. G: Average mass fraction of the NAFs in the different scenarios. H: Time evolution of the mass fraction profile of the cut of the simulation window in the case of a tumor in the presence of efficient T-cells and NAFs only, without T-cells.
Different tumor phenotypes in non-spherical domains.
Deformed shapes result in anisotropic configurations by forcing NAFs and T-cells to enter the domain from only one side of the boundary. Different colors indicate separated regions where the nest (in light blue) is confined by the boundary of CAFs (in red) within a healthy stroma (in green). A: Immune-inflamed case leads to disappearance cancer cells at t = 5T0. B: Elliptical area leads to slower dynamics.
