Figures and data
The overall degradation rates of soil extracellular 16S rRNA genes and their influential factors.
(a) The GAPDH F-labeled 16S rRNA gene copies at different incubation time points; (b) the degradation rates of soil extracellular 16S rRNA genes across the study sites; (c) the factors influencing extracellular 16S rRNA gene degradation rates; and (d) the influencing factors for the overall degradation rates of soil extracellular 16S rRNA genes revealed by structural equation modeling. Orange and green lines indicate positive and negative relationships, respectively. Solid and dashed lines indicate significant and non-significant relationships, respectively. Path coefficients are denoted by numbers adjacent to the arrows, with arrow width reflecting their strength. Significance levels are indicated as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001. Moisture: soil moisture content; Abundance: prokaryotic abundance; NMDS1: the scores at the first axis of the NMDS ordination of prokaryotic community profiles; MAP: mean annual precipitation; Richness: soil prokaryotic richness; NMDS2: the scores at the second axis of the NMDS ordination of prokaryotic community profile; TK: soil total potassium contents; MAT: mean annual temperature; AP: soil available phosphorus content; TN: soil total nitrogen contents; and TOC: soil total organic carbon content.
The sequence-specific degradation rates of soil exogenous extracellular 16S rRNA genes.
(a) Soil GAPDH F-labeled 16S rRNA gene richness; (b) the nonmetric multidimensional scaling (NMDS) ordination of the community profiles based on GAPDH F-labeled 16S rRNA genes at different incubation time points; (c) the number of GAPDH F-labeled 16S rRNA gene ASVs within different degradation rates range
The diffrence in sequence-specific degradation rates of soil exogenous extracellular 16S rRNA genes and their influencing factors.
(a) the paired comparison of degradation rates among different AVSs. Blue indicates that the degradation rate of the ASV I group is lower than that of the ASV II group, and the T value is negative; red indicates that the degradation rate of the ASV I group is higher than that of the ASV II group, and the T value is positive. Significance levels are indicated as follows: * P < 0.05, ** P < 0.01, and *** P < 0.001. and (b) the relationships between the sequence-specific degradation rate profiles and environmental factors. NO --N: soil NO --N contents; NH +-N: soil NH +-N contents; TN: soil total N contents; TP: soil total P contents; TK: soil total K contents; AP: soil available P contents; TOC: soil total organic carbon content; MAT: mean annual temperature; and MAP: mean annual precipitation.
The disparities in the abundance, richness, and community composition between total and living soil prokaryotes.
(a) The abundance of total and living soil prokaryotes. (b) The richness of total and living soil prokaryotes. (c) The differences between the relative abundance of total and intracellular ASVs. The red points represent the prokaryotic taxa exhibiting statistically significant differences. (d) The nonmetric multidimensional scaling (NMDS) ordination of total and living soil prokaryotes. The different colors represent samples from different sites.
(a) The taxa with significant differences between total and living prokaryotic communities. P1: Thermoplasmatota; P2: Nitrospirota; P3:, Fibrobacterota; P4: Entotheonellaeota; P5: Elusimicrobiota; P6: Armatimonadota; P7: Myxococcota; C1: Thermoplasmata; C2: Nitrospiria; C3: Vampirivibrionia; C4: Clostridia; C5: Bacilli; C6: Armatimonadia; C7: Chthonomonadetes; C8: Polyangia; C9: Blastocatellia; C10: Parcubacteria; C11: Bacteroidia; C12: Thermoleophilia; O1: Nitrospirales; O2: Abditibacteriales; O3: Clostridiales; O4: Bacillales; O5: Armatimonadales; O6: Pyrinomonadales; O7: Bryobacterales; O8: Chitinophagales; O9: Rubrobacterales; O10: Propionibacteriales; and O11: Corynebacteriales; (b) the relationships between soil prokaryotic community profiles and environmental factors. NO --N: soil NO --N contents; NH +-N: soil NH +-N contents; Moisture: soil moisture content; MAT: mean annual temperature; MAP: mean annual precipitation; TP: soil total P contents; TK: soil total K contents; and TOC: soil total organic carbon contents.
