Effects of atg RNAi on longevity are highly variable and condition dependent.

(A) The autophagy pathway, and genes tested in this study. (B-D) Effects at 20°C. (B) Effects on N2 (wild type). (C) Effects on daf-2(e1368). (D) Effects on daf-2(e1370). (F, G) Effects at 25°C. (F) Effects on N2. (G) Effects on daf-2(e1368). (H) Effects of bec-1 RNAi on daf-2(e1370) at 15°C. (B-G summed data, N = 2; for individual trials and statistical comparisons, see Table S2, S5.

Degree of suppression of daf-2 longevity by atg gene RNAi differs greatly between daf-2 alleles and atg RNAi treatments (20°C), cf. Figure 1B-D (same data).

Summed data, N = 2; for individual trials and statistical comparisons, see Table S2. All trials were performed in parallel, i.e. all lifespans are directly comparable.

FUDR but not infection alters outcome on atg gene RNAi (20°C).

(A-C) Effects of 0 μM, 15 μM and 800 µM FUDR. (D) No alteration by kanamycin of atg-13 RNAi effect on N2 lifespan. Summed data, N = 2; for individual trials and statistical comparisons, see Table S6, S7.

Degree of suppression of glp-1(e2141) longevity by atg gene RNAi differs greatly between genes.

(A, B) 20°C. (A) Effects on N2. (B) Effects on glp-1. (C, D) 25°C. (C) Effects on N2. (D) Effects on glp-1. Summed data, N = 2-5; for individual trials and statistical comparisons, see Table S9. (E) Overview of effects of suppression of Age by RNAi of genes specifying autophagy. Dark blue, reduction of daf-2 or glp-1 Age that is significantly greater than in N2. Light blue, reduction of daf-2 or glp-1 Age that is not significantly greater than in N2. R, robust suppression, i.e. strong but incomplete suppression of longevity, defined here as <30% increase in mean lifespan in daf-2 or glp-1 relative to N2, with RNAi, and as evident from Figure 2, Figure 5, and Fig. S2.

Degree of suppression of glp-1(e2141) longevity by atg gene RNAi differs greatly between genes (20°C), cf. Figure 4A,B (same data).

Summed data, N = 2-5; for individual trials and statistical comparisons, see Table S9.

Absence of effect of atg RNAi on vitellogenin accumulation.

Fold change of yolk proteins YP170, YP115 and YP88 in N2 and fog-2 normalized to gfp day 1 (N = 3). In no case is vitellogenin level significantly different to that in the gfp RNAi control at any time point (two-way ANOVA, Table S10). For raw data for vitellogenin levels see Supplementary Dataset 3.