CMC and alkali treatment facilitate detection of Ψ, D, and m7G modifications in E. coli.
(A, B) Heatmaps of the misincorporation signals of E. coli tRNAs treated with (A) or without (B) CMC. In both conditions, tRNAs are incubated in alkali condition. Known Ψ, D, and m7G sites are shown. Ψ are shown in white. (C) Misincorporation frequency at known Ψ, D, m7G sites of tRNAs treated with CMC+/alkali+ or CMC-/alkali+, and tRNAs without treatment.