The pair-stimulations of the whisker stimulus (WS) with the odor stimulus (OS), the tail-heating stimulus (TS) and the gustatory stimulus (GS) sequentially lead to odorant-induced whisker motion versus whisking-induced olfactory response, tail-heating-induced whisker motion versus whisking-induced tail swing, and gustation-induced whisker motion versus whisking-induced taste response. (A) The associative learning in C57BL/6JThy1-YFP mice was conducted by the pair-stimulations of the whisker tactile signal with the olfactory butyl acetate signal, the whisker tactile signal with the sucrose taste signal and the whisker tactile signal with the tail-heating signals sequentially in mice, which were assigned in paired-stimulus group (PSG), compared to mice in unpair-stimulus group (UPSG), for 12 days. (B, D, F) The OS, the TS and the GS appear to induce whisker motions in a fluctuation pattern in PSG mice, respectively, but not in UPSG mice. Calibration bars are 30°of whisker deflection and 5 seconds. The statistical analyses in right panels show whisking amplitudes in response to the OS, TS and GS in PSG mice (red bar) and in UPSG mice (blue bar). (C) As indicated by the moving trace, PSG mice prefer to move away from the butyl acetate side as the olfactory response to the whisker stimulus compared with the UPSG mice (left panel). The percentage of avoidance of butyl acetate in PSG mice (red bar, right panel) and in UPSG mice (blue bar). (E) The WS appears to induce tail swing after training in PSG, but not in UPSG mice (left panel). The statistical analyses about tail swing time in response to the WS in PSG mice (red bar, right panel) and in UPSG mice (blue bar). (G) The WS appears to induce mouth movements after training in PSG mice, but not in UPSG mice (left panel). The statistical analyses about number of mouth movements in response to the WS in PSG mice (red bar, right panel) and in UPSG mice (blue bar).