Comparison of manual gating (manual1 and manual2), BinaryClust and flowSOM clustering results in MPN cohort (n=9). Interaction plots showing the individual measurement (percentage) of each study participant with indicated colors by different methods across main cell lineages (B cells, CD8 T cells, Gamma Delta T cells, NK cells, Dendritic cells, monocytes and CD4 T cells); ANOVA was used for statistical testing, and significance was marked by asterisk. * P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.