The differentiation of liver macrophages was inhibited in Bmp9fl/flBmp10fl/flLratCre mice.
(a,b) PCA and volcano plot of the RNA-seq data of sorted liver macrophages from Bmp9fl/flBmp10fl/flLratCre and control mice at the age of 8-10 weeks (n=3/group). Genes upregulated and downregulated are shown in red and blue, respectively (fold change [FC] > 2, adjusted p[p-adj] < 0.05).
(c) Heatmap showing signature genes expressed differentially in liver macrophages from Bmp9fl/flBmp10fl/flLratCre and control mice.
(d) Expression counts of indicated genes in liver macrophages from Bmp9fl/flBmp10fl/flLratCre and control mice.***p-adj < 0.001.
(e) Immunofluorescence images of F4/80+ and CD64+ liver macrophages in sections from Bmp9fl/flBmp10fl/flLratCre mice and their controls at the age of 12 weeks (n=5/group). Liver macrophages number was measured (right).
(f) Representative flow cytometric expression of CLEC2 and TREML4 in liver macrophages from the indicated mice at the age of 8-12 weeks (n=3-4/group).
(g,h) PCA and volcano plot of the RNA-seq data of sorted liver macrophages from Smad4fl/flVav1Cre and control mice at the age of 8-10 weeks (n=3/group). Genes upregulated and downregulated are shown in red and blue, respectively (fold change [FC] > 2, adjusted p[p-adj] < 0.05).
(i) Heatmap showing signature genes expressed differentially in liver macrophages from Smad4fl/flVav1Cre and control mice.
(j, k) Venn diagram showing DE genes (j) and transcription factors (k) specific to liver macrophages of Bmp9fl/flBmp10fl/flLratCre, Smad4-deficient liver macrophages or shared between both mac populations. Results represent the mean ± SEM. *P < 0.05, by Mann-Whitney test (e).