Remyelination by FAP-derived BDNF during peripheral nerve regeneration.
(A) Experimental scheme displaying mice used and the time points selected for EMG measurements and sciatic nerve dissection. wpi, weeks post injury. See Figure 5—figure supplement 1D for the EMG measurement scheme. (B) Representative EMG measurement results of both injured and uninjured GA muscles from Ctrl or cKO mice at the indicated time points post SNC. (C–D) Quantified results of EMG measurement showing (C) CMAP amplitude and (D) CMAP latency. n = 5. One-way ANOVA with Bonferroni’s post hoc test. * p < 0.05, ** p < 0.01, *** p < 0.001, n.s., not significant. See Figure 5—figure supplement 1E for the EMG measurement quantification metholodogy. (E) Representative images showing toluidine blue-stained, semi-thin cross-sections of sciatic nerves dissected from Ctrl or cKO mice at 6 wpi. Scale bars, 10 μm. (F–G) Quantification of (F) calculated G-ratio values and (G) axon diameters from analyzing toluidine blue-stained sciatic nerve sections dissected from Ctrl or cKO mice at 6 wpi. 50 axons were randomly selected from each sciatic nerve for quantification. n = 5. Mann-Whitney U test. *** p < 0.001, n.s., not significant. See Figure 5—figure supplement 1F and G for G-ratio quantification scheme and methodology.
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