gal Controls Whole-Brain Activity
A) Representative calcium signals (elavl3:GCaMP5G) recorded across the brain of 5 dpf control (blue, top) and hsp70l:gal sibling (orange, bottom). B) AUC calculated over 1 h recording, normalized to control (n control = 25; n hsp70l:gal = 29). C) Galanin transcript levels in pools of control vs. hsp70l:gal brains (n = 3). D) Number of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right). E) Average amplitude of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right) per larva. F) Average duration of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right) per larva. G) Representative calcium signals (elavl3:GCaMP5G) recorded across the brain of 5 dpf control (blue, top) and gal-/- larva (yellow, bottom). H) AUC calculated over 1 h recording, normalized to control (n control = 24; n gal-/- = 24). I) Number of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right). J) Average amplitude of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right) per larva. K) Average duration of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right) per larva. L) Representative calcium signals (elavl3:GCaMP6f) recorded across the brain of 5 dpf control injected (blue, top) and galr1a crispant (galr1aKO) larva (green, bottom). M) AUC calculated over 1 h recording, normalized to control (n control = 28; n galr1a crispant = 23). N) Number of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right). O) Average amplitude of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right) per larva. P) Average duration of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right) per larva. Significance levels: ***p < .001, **p < .01, *p < .05, ns = not significant (p > .05), Wilcoxon-Mann-Whitney test (B, E, F, H, J, K, M, O, P), negative binomial regression (D, I, N), Student’s t-test (C).