Cyanobacteria cope with both predictable day/night changes and natural fluctuations in light during the day by adjusting the expression dynamics of circadian-clock-controlled genes via a network of transcriptional regulators.
During Staphylococcus aureus infections, bacterial cells bifurcate into distinct, specialized cell types that localize physically in different colonization tissues to simultaneously generate different infection types.
Live-cell imaging, genetic analysis and electron cryomicroscopy identify structural motifs involved in the differential assembly of Pol I-Rrn3 complexes and Pol I homodimers in response to nutrient availability.
Cryo-electron microscopy structures, combined with biochemical experiments, show how the E. coli F element-encoded TraR protein regulates transcription initiation by altering RNA polymerase conformation and conformational heterogeneity.
Investigation of global gene expression profiles during formation of the Myxococcus xanthus specialized biofilm reveals a genetic regulatory network that coordinates cell motility, differentiation, and secondary metabolite production.
Biochemical analyses of transcription complexes, including kinetic studies and probes of translocational and conformational states, establish the elemental mechanism of pausing, which underlies regulation of gene expression in all organisms.