Forward genetics and super-resolution microscopy identifies ZitP as a conserved multifunctional regulator that accumulates at both cell extremities in distinct macromolecular structures to perform different functions in the asymmetric model bacterium Caulobacter crescentus.
Cryo-electron microscopy structures, combined with biochemical experiments, show how the E. coli F element-encoded TraR protein regulates transcription initiation by altering RNA polymerase conformation and conformational heterogeneity.
Biochemical analyses of transcription complexes, including kinetic studies and probes of translocational and conformational states, establish the elemental mechanism of pausing, which underlies regulation of gene expression in all organisms.
Investigation of global gene expression profiles during formation of the Myxococcus xanthus specialized biofilm reveals a genetic regulatory network that coordinates cell motility, differentiation, and secondary metabolite production.
A riboswitch class has been discovered for phosphoribosyl pyrophosphate, the biochemical precursor of nucleotides, and this novel RNA sensor commonly operates in tandem with a guanine aptamer to function like a Boolean logic gate.