Procedures were developed to perform mesoscale 2-photon Ca²⁺ imaging simultaneously from all of mouse dorsolateral neocortex, facilitating identification of widespread neural ensembles with activity related to diverse aspects of behavior.

Simultaneous 2-photon imaging of striosomes and matrix in mice shows that striosomes preferentially encode reward-predicting cues whereas both striatal compartments demonstrate reward-related activity.

Improved two-photon fluorescence microendoscopy enables volumetric imaging of synapses and neurons in deep brain structures in vivo.

Quantitative experiments and analysis determine the limit of excitation power of 1300-nm three-photon microscopy, and the imaging depth where three-photon outperforms two-photon for calcium imaging in the mouse brain.

Development of imaging methods to capture neural activity and structure through the intact cuticle using 2-photon and 3-photon excitation in the genetic model organism, Drosophila melanogaster.

Aberration correction using 3D microprinting in ultrathin microendoscopes allows two-photon imaging of large neuronal networks with homogeneously high spatial resolution and minimal invasiveness in the deep mouse brain.

Sensory enrichment creates a more columnar, less salt-and-pepper whisker map in somatosensory cortex, showing that impoverished experience contributes to intermixed tuning in rodent sensory maps.

A combination of optogenetics and 2-photon calcium imaging reveals spatial prerequisites for non-linear synaptic summation within a defined cortical connection.

Two-photon Ca²⁺ imaging and computational modeling reveal major developmental trajectories of spontaneous activity in developing CA1 and identify important roles of network bi-stability and synaptic input characteristics for hippocampal burstiness before eye opening.

Two-photon functional imaging linked to olfactory preference in Drosophila provides the first understanding of how odors are integrated, transformed, and represented in the lateral horn by an ensemble of higher-order glutamatergic lateral horn neurons.