3,141 results found
    1. Structural Biology and Molecular Biophysics

    2.8 Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy

    Melody G Campbell et al.
    Water molecules and amino-acid side chain rotamers have been resolved in the cryoEM structure of T20S proteasome at 2.8 angstroms.
    1. Biochemistry and Chemical Biology

    Specific lid-base contacts in the 26s proteasome control the conformational switching required for substrate degradation

    Eric R Greene et al.
    The 26S proteasome lid subcomplex acts as an external scaffold whose interactions with the ATPase motor stabilize the substrate-engagement-competent state, and control conformational changes upon engagement for subsequent degradation steps.
    1. Structural Biology and Molecular Biophysics

    Atomic structure of the 26S proteasome lid reveals the mechanism of deubiquitinase inhibition

    Corey M Dambacher et al.
    Within the isolated lid sub-complex of the proteasome, a finely tuned network of interactions maintains the deubiquitinase in an inhibited conformation; dramatic rearrangements of the lid subunits upon incorporation into the holoenzyme lead to the deubiquitinase’s activation.
    1. Structural Biology and Molecular Biophysics

    Model-based local density sharpening of cryo-EM maps

    Arjen J Jakobi et al.
    Local sharpening of cryo-electron microscopy maps using atomic models can increase the interpretability of densities in cases of resolution variation and facilitates model building and refinement.
    1. Structural Biology and Molecular Biophysics

    Cryo-EM single particle analysis with the Volta phase plate

    Radostin Danev, Wolfgang Baumeister
    The Volta phase plate enables in-focus single particle analysis at near atomic resolutions, which could expand the capabilities of cryo-EM, especially for small, flexible or heterogeneous macromolecular samples.
    1. Cell Biology

    Compromising the 19S proteasome complex protects cells from reduced flux through the proteasome

    Peter Tsvetkov et al.
    Reducing the expression of 19S subunits shifts the ratio of 20S/26S proteasome complexes and provides a powerful survival advantage in the face of lethal proteasome inhibition.
    1. Structural Biology and Molecular Biophysics

    Measuring the optimal exposure for single particle cryo-EM using a 2.6 Å reconstruction of rotavirus VP6

    Timothy Grant, Nikolaus Grigorieff
    The resolution of electron cryo-microscopy can be improved by using optimal exposure values to filter video frames.
    1. Cell Biology
    2. Immunology and Inflammation

    Multiplexed proteomics of autophagy-deficient murine macrophages reveals enhanced antimicrobial immunity via the oxidative stress response

    Timurs Maculins et al.
    Multiplexed mass spectrometry shows how the autophagy gene Atg16l1 regulates macrophage innate immunity against intracellular bacterial pathogens by modulating basal oxidative stress responses.
    1. Structural Biology and Molecular Biophysics
    2. Computational and Systems Biology

    Molecular dynamics-based refinement and validation for sub-5 Å cryo-electron microscopy maps

    Abhishek Singharoy et al.
    New hybrid structure determination methods leveraging the inherent biophysical properties of a macromolecule through molecular dynamics simulations provide accurate and cost-efficient ways of achieving atomic structures from high resolution cryo-electron density maps.
    1. Cell Biology

    Regulator of G protein signaling 12 enhances osteoclastogenesis by suppressing Nrf2-dependent antioxidant proteins to promote the generation of reactive oxygen species

    Andrew Ying Hui Ng et al.
    Rgs12 protein is a novel regulator of the Nrf2 protein by facilitating the proteasomal degradation of the transcription factor during osteoclast differentiation.

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