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    1. Structural Biology and Molecular Biophysics
    2. Developmental Biology

    Quantitative imaging reveals real-time Pou5f3–Nanog complexes driving dorsoventral mesendoderm patterning in zebrafish

    Mireia Perez-Camps, Jing Tian ... Bruno Reversade
    Different combinations of the transcription factors Pou5f3, Nanog and Sox32 form complexes to drive dorsoventral mesendoderm patterning in zebrafish.
    1. Computational and Systems Biology
    2. Developmental Biology

    Noise modulation in retinoic acid signaling sharpens segmental boundaries of gene expression in the embryonic zebrafish hindbrain

    Julian Sosnik, Likun Zheng ... Thomas F Schilling
    Direct measurements of retinoic acid using fluorescence lifetime imaging reveal a new role for cellular retinoic acid binding proteins in noise attenuation that is critical to sharpen hindbrain rhombomere boundaries.
    1. Neuroscience

    Retinal microvascular and neuronal pathologies probed in vivo by adaptive optical two-photon fluorescence microscopy

    Qinrong Zhang, Yuhan Yang ... Na Ji
    A properly designed two-photon fluorescence microscope allows high-resolution visualization of neurons and capillaries in healthy and diseased mouse retinas in vivo, while to visualize subcellular structures, adaptive optics is required to correct the eye-induced optical aberrations.
    1. Microbiology and Infectious Disease

    The flagellar motor of Vibrio alginolyticus undergoes major structural remodeling during rotational switching

    Brittany L Carroll, Tatsuro Nishikino ... Jun Liu
    In situ structures of the flagellar motors genetically docked in CCW and CW rotational states reveal major rearrangement that facilitates rotational switch in Vibrio alginolyticus..
    1. Neuroscience

    Co-agonists differentially tune GluN2B-NMDA receptor trafficking at hippocampal synapses

    Joana S Ferreira, Thomas Papouin ... Laurent Groc
    D-serine has a major role in the regulation of NMDA receptors not only contributing to its activation as the receptors co-agonist, but also by regulating specifically GluN2B-NMDA receptor trafficking and synaptic content at developing hippocampal synapses.
    1. Cell Biology

    Symmetric exchange of multi-protein building blocks between stationary focal adhesions and the cytosol

    Jan-Erik Hoffmann, Yessica Fermin ... Eli Zamir
    In the cytosol, the proteins constituting cell-matrix adhesion sites form multi-protein building blocks which enter and leave these sites unaltered, thereby contributing to their rapid and correct self-assembly.
    1. Cell Biology
    2. Neuroscience

    The promise and peril of comparing fluorescence lifetime in biology revealed by simulations

    Pingchuan Ma, Scott Sternson, Yao Chen
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    1. Cancer Biology
    2. Cell Biology

    Bim escapes displacement by BH3-mimetic anti-cancer drugs by double-bolt locking both Bcl-XL and Bcl-2

    Qian Liu, Elizabeth J Osterlund ... David William Andrews
    The pro-apoptotic BH3-protein Bim contains two distinct binding sites for anti-apoptotic proteins that together confer resistance of Bim/Bcl-2 and Bim/Bcl-XL complexes to BH3-mimetic drugs under development for use in humans.
    1. Neuroscience

    Rab10 inactivation promotes AMPAR insertion and spine enlargement during long-term potentiation

    Jie Wang, Jun Nishiyama ... Ryohei Yasuda
    Not revised
    Reviewed Preprint v1
    • Important
    • Solid
    • Incomplete
    1. Neuroscience

    Computer assisted detection of axonal bouton structural plasticity in in vivo time-lapse images

    Rohan Gala, Daniel Lebrecht ... Armen Stepanyants
    BoutonAnalyzer software makes it possible to detect axonal boutons and measure their structural plasticity in long-term in vivo imaging experiments.