Melanin staining for micro-CT imaging, using a novel application of ionic silver deposition, enables qualitative and quantitative 3D analysis of zebrafish pigmentation and reveals subtle phenotypes missed by light microscopy.

Lattice light-sheet single-molecule imaging shows 3D Sox2 enhancer clusters in live embryonic stem cells and reveals a model linking 3D spatial distribution of cis-elements, differential target search features and localized gene regulation.

Complete neuronal circuits can be reconstructed, and whole eukaryotic cells imaged, at less than 10 nm isotropic resolution.

Three-dimensional fluorescence imaging of microbial eukaryotes in environmental samples allows accurate automated taxonomic profiling and quantitative data about ultrastructures and interactions of organisms.

By integrating multiplexed error-robust fluorescence in situ hybridization (MERFISH), deep learning, optical sectioning, and protocol optimization, thick-tissue 3D MERFISH allows volumetric single-cell transcriptome imaging of thick-tissue samples, broadening the scope of applications of spatial genomics.

A one-photon three-dimensional light sculpture technique has been developed and demonstrated for high spatio-temporal resolution photostimulation and imaging of dozens of neurons in the intact mouse brain in vivo.

A deep learning-based pipeline was developed for extracting cellular signals flexibly from moving cells in 3D time lapse images, and it outperformed previous methods under different imaging conditions.

Fertilizing mouse spermatozoa, characterized by intact CatSper channels, lack of protein tyrosine phosphorylation, and reacted acrosomes, in the female reproductive tract provide molecular insight into sperm selection for successful fertilization.

The integration of a novel high spatiotemporal resolution volume imaging technique and a fast 3D tracking system allows capturing whole brain neural activities in a freely behaving larval zebrafish.

An all-optical 3D two-photon imaging and photostimulation platform was demonstrated, with the capability to precisely stimulate a large group of cells in mice cortex in vivo with low laser power.