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    1. Cell Biology

    Glucokinase activity controls peripherally located subpopulations of β-cells that lead islet Ca2+ oscillations

    Erli Jin, Jennifer K Briggs ... Matthew J Merrins
    3D light-sheet imaging identifies the location and metabolic sensitivity of β-cell subpopulations that lead and coordinate islet Ca2+ oscillations.
    1. Cell Biology
    2. Neuroscience

    Complete morphologies of basal forebrain cholinergic neurons in the mouse

    Hao Wu, John Williams, Jeremy Nathans
    The first complete 3D structures of mouse cholinergic neurons reveal the length and complexity of their axons, while calculations suggest that equivalent axons in the human brain are approximately than 100 meters long.
    1. Cell Biology

    A disassembly-driven mechanism explains F-actin-mediated chromosome transport in starfish oocytes

    Philippe Bun, Serge Dmitrieff ... Péter Lénárt
    On entry to meiosis, chromosomes scattered in the large oocyte nucleus of starfish are collected by an F-actin network, which contracts by an unexpected, myosin-independent mechanism based on local assembly and global disassembly of actin filaments.
    1. Developmental Biology

    Translational rapid ultraviolet-excited sectioning tomography for whole-organ multicolor imaging with real-time molecular staining

    Wentao Yu, Lei Kang ... Terence TW Wong
    TRUST, as a rapid and fully automated whole-organ imaging technique, enables high-resolution multicolor 3D imaging at low cost by using a UV-LED for widefield illumination and a color camera while relieving researchers from lengthy and laborious tissue processing protocols.
    1. Computational and Systems Biology
    2. Neuroscience

    The geometry of robustness in spiking neural networks

    Nuno Calaim, Florian A Dehmelt ... Christian K Machens
    Spiking neural networks become robust to various perturbations of their parameters if their voltages are confined to a lower-dimensional subspace, and both dynamics and robustness can be visualised in this voltage subspace.
    1. Computational and Systems Biology
    2. Developmental Biology

    A versatile pipeline for the multi-scale digital reconstruction and quantitative analysis of 3D tissue architecture

    Hernán Morales-Navarrete, Fabián Segovia-Miranda ... Yannis Kalaidzidis
    MotionTracking is freely available software that can generate multi-scale geometrical models of mammalian tissue and perform quantitative analysis of tissue architecture.
    1. Neuroscience
    2. Physics of Living Systems

    Replay as wavefronts and theta sequences as bump oscillations in a grid cell attractor network

    Louis Kang, Michael R DeWeese
    An experimentally supported model of grid cells naturally enables them to participate in firing sequences that encode rapid trajectories in space.
    1. Neuroscience
    2. Physics of Living Systems

    Revealing architectural order with quantitative label-free imaging and deep learning

    Syuan-Ming Guo, Li-Hao Yeh ... Shalin B Mehta
    Advances in quantitative phase and polarized light microscopy, combined with deep learning, reveal the architecture of human brain tissue.
    1. Neuroscience
    2. Physics of Living Systems

    Gigapixel imaging with a novel multi-camera array microscope

    Eric E Thomson, Mark Harfouche ... Eva A Naumann
    A new multi camera imaging platform simultaneously captures large-area, high-resolution video of unconstrained small model organisms and provides behavioral measurements that span multiple spatial scales.
    1. Neuroscience

    Ultrastructural heterogeneity of layer 4 excitatory synaptic boutons in the adult human temporal lobe neocortex

    Rachida Yakoubi, Astrid Rollenhagen ... Joachim HR Lübke
    Neocortical synapses in layer 4 of the human temporal lobe neocortex were quantitatively characterized, at the subcellular level, using high-end, high-resolution electron microscopy and 3D-volume reconstructions.