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    1. Chromosomes and Gene Expression

    A simple method for generating high-resolution maps of genome-wide protein binding

    Peter J Skene, Steven Henikoff
    Building on previous work (Skene et al., 2014), we show that a new ChIP-seq protocol provides superior resolution and ease of use at low sequence depth of coverage for generating genome-wide maps of protein binding.
    1. Structural Biology and Molecular Biophysics

    High-resolution and high-accuracy topographic and transcriptional maps of the nucleosome barrier

    Zhijie Chen et al.
    Direct observation of RNA Polymerase II transcription through a single nucleosome at near basepair resolution suggests a mechanism for selective control of gene expression.
    1. Structural Biology and Molecular Biophysics
    2. Developmental Biology

    Quantitative imaging reveals real-time Pou5f3–Nanog complexes driving dorsoventral mesendoderm patterning in zebrafish

    Mireia Perez-Camps et al.
    Different combinations of the transcription factors Pou5f3, Nanog and Sox32 form complexes to drive dorsoventral mesendoderm patterning in zebrafish.
    1. Chromosomes and Gene Expression

    Yeast Nuak1 phosphorylates histone H3 threonine 11 in low glucose stress by the cooperation of AMPK and CK2 signaling

    Seunghee Oh et al.
    Yeast Nuak1 is an AMPK and CK2 dependent H3 T11 kinase.
    1. Neuroscience

    Plasticity of calcium-permeable AMPA glutamate receptors in Pro-opiomelanocortin neurons

    Shigetomo Suyama et al.
    Neurons in the hypothalamus that promote satiety manifest plasticity of their response to the excitatory transmitter, glutamate, by re-organizing the composition of a specific glutamare receptor.
    1. Biochemistry and Chemical Biology
    2. Computational and Systems Biology

    Snf1/AMPK promotes the formation of Kog1/Raptor-bodies to increase the activation threshold of TORC1 in budding yeast

    James E Hughes Hallett et al.
    The key Target of Rapamycin Complex (TORC1) component Kog1 moves into bodies during glucose starvation to limit reactivation of the complex.
    1. Cell Biology

    Metformin extends C. elegans lifespan through lysosomal pathway

    Jie Chen et al.
    Metformin extends C. elegans lifespan through lysosome-dependent coordination of TORC1 and AMPK pathways.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Nucleosome-CHD4 chromatin remodeler structure maps human disease mutations

    Lucas Farnung et al.
    The structure of the chromatin remodeller CHD4 bound to a nucleosome reveals differences to the known Chd1-nucleosome complex and maps cancer mutations.
    1. Structural Biology and Molecular Biophysics
    2. Neuroscience

    Preferential assembly of heteromeric kainate and AMPA receptor amino terminal domains

    Huaying Zhao et al.
    Members of kainate and AMPA families of glutamate receptors exhibit large differences in affinity for homo- and hetero-dimerziation of their amino terminal domains, which may control gating characteristics and ion selectivity of receptor subtypes with different subunit composition.
    1. Structural Biology and Molecular Biophysics

    Model-based local density sharpening of cryo-EM maps

    Arjen J Jakobi et al.
    Local sharpening of cryo-electron microscopy maps using atomic models can increase the interpretability of densities in cases of resolution variation and facilitates model building and refinement.