The structure of the chromatin remodeller CHD4 bound to a nucleosome reveals differences to the known Chd1-nucleosome complex and maps cancer mutations.
A technology to produce homogenously poly-ADP-ribosylated proteins reveals key molecular mechanisms that govern ATP-dependent chromatin remodeling activity at DNA damage sites.
Chromatin remodeling enzymes perform target search by utilizing ATP-binding and hydrolysis to facilitate 1D-diffusion on and rapid detachment from chromatin in living yeast.
Ben C Tilly, Gillian E Chalkley ... C Peter Verrijzer
Visualization of BRM remodelers engaging their endogenous genomic targets revealed highly transient and dynamic interactions with chromatin, which are fueled by ATP-hydrolysis.
Single-particle imaging of RSC and ISW2 chromatin remodelers on DNA stretched between dual optical tweezers reveals distinctive 1D diffusion and opposing push–pull nucleosome translocation on engagement with sparse nucleosome arrays.
Alisha Schlichter, Margaret M Kasten ... Bradley R Cairns
A SWI/SNF-family chromatin remodeling subcomplex from yeast is identified (RSC1) that is specialized to slide nucleosomes residing on DNA sequences that confer partial nucleosome unwrapping, with assistance from accessory factors.
Unlike Chd1, ISWI remodelers preferentially shift asymmetric nucleosomes toward the side possessing a wild type acidic patch, regardless of DNA lengths flanking the nucleosome.
The chromatin remodeller BRG1 is recruited to pluripotency-associated gene regulatory elements by the pioneer transcription factor OCT4 to support further transcription factor binding and gene regulation.
