The crenarchaeon Sulfolobus synthesizes the N-glycan core in the identical way as all Eukaryotes, which strengthens the hypothesis that the eukaryotic N-glycosylation is acquired from an ancient archaeon during eukaryogenesis.

TOG (tumor overexpressed gene) domains organize then polymerize tubulins at microtubule plus ends.

Upon AMPK activation by acute glucose restriction, Atg14p shifts its distribution from ER exit sites to liquid-ordered membrane domains on the vacuolar surface where, together with Atg6p, it facilitates LD-specific autophagy (i.e., microautophagy of lipid droplets) for energy production and long-term survival of starved cells.

A cell-free biochemical assay for protein lipidation identifies the ER–Golgi intermediate compartment as a key early station in the formation of an autophagosome.

Notch1 activates autophagy to regulate the survival of T-regulatory cells from mice.

Building on previous work (Ge et al., 2013), it is shown that the ER-Golgi intermediate compartment is a platform for the production of COPII vesicles as precursor membranes for the lipidation of LC3, which is an essential step in autophagosome biogenesis.

Nutrient starvation activates autophagy by a novel signaling mechanism that is blocked by one of the major autophagy inhibitors.

The first three-dimensional structure of the PI3KC3 complex I, which is involved in autophagy, provides a framework for understanding the allosteric regulation of lipid kinase activity.