48 results found
    1. Structural Biology and Molecular Biophysics
    2. Neuroscience

    Single-molecule fluorimetry and gating currents inspire an improved optical voltage indicator

    Jeremy S Treger, Michael F Priest, Francisco Bezanilla
    ArcLight, a popular optogenetic reporter of voltage, is studied at both single-molecule and macroscopic levels, which leads to new mechanistic understanding and to the rational design of a faster reporter.
    1. Neuroscience

    Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators

    Simon Chamberland, Helen H Yang ... François St-Pierre
    The combination of a new genetically encoded voltage indicator and fast two-photon imaging methods enables detection of rapid neural electrical activity in organotypic slice cultures and in living flies.
    1. Neuroscience

    Hunger- and thirst-sensing neurons modulate a neuroendocrine network to coordinate sugar and water ingestion

    Amanda J González Segarra, Gina Pontes ... Kristin Scott
    A peptidergic network translates internal nutrient abundance cues into the coordinated regulation of sugar and water ingestion.
    1. Neuroscience

    Excitation and inhibition onto central courtship neurons biases Drosophila mate choice

    Benjamin R Kallman, Heesoo Kim, Kristin Scott
    Pheromones activate excitatory and inhibitory pathways that are integrated to guide mating decisions in Drosophila melanogaster.
    1. Neuroscience

    A single pair of neurons links sleep to memory consolidation in Drosophila melanogaster

    Paula R Haynes, Bethany L Christmann, Leslie C Griffith
    Dorsal paired medial neurons, which are required for memory consolidation, promote sleep by inhibiting the excitation of mushroom body neurons.
    1. Neuroscience

    Distinct combinations of variant ionotropic glutamate receptors mediate thermosensation and hygrosensation in Drosophila

    Zachary A Knecht, Ana F Silbering ... Paul A Garrity
    A combination of genetics, behavior and physiology demonstrate that cool and moisture sensing rely on overlapping combinations of sensory receptors in Drosophila.
    1. Biochemistry and Chemical Biology

    Probing PAC1 receptor activation across species with an engineered sensor

    Reto B Cola, Salome N Niethammer ... Tommaso Patriarchi
    A new genetically encoded sensor robustly detects exogenously applied PAC1R ligands in vitro, ex vivo, and in vivo (in zebrafishes and mice) serving as a useful resource for probing PAC1R functions.
    1. Neuroscience

    Drosophila gustatory projections are segregated by taste modality and connectivity

    Stefanie Engert, Gabriella R Sterne ... Kristin Scott
    Anatomical and synaptic reconstructions of gustatory axons from the adult Drosophila labellum reveal different classes of gustatory neurons recognizing different taste modalities.
    1. Neuroscience

    Ionotropic Receptor-dependent moist and dry cells control hygrosensation in Drosophila

    Zachary A Knecht, Ana F Silbering ... Paul A Garrity
    The neurons and receptors mediating moist air detection in Drosophila are identified, revealing that moist and dry air detection depend on overlapping Ionotropic Receptors and that these pathways are both critical for hygrosensation.
    1. Neuroscience

    The role of PDF neurons in setting the preferred temperature before dawn in Drosophila

    Xin Tang, Sanne Roessingh ... Fumika N Hamada
    In Drosophila, key neurons controlling sleep play an important role in adjusting the temperature set-point before dawn via time-dependent neuronal plasticity within the clock circuits.

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