Diverse sophisticated phylogenetic analyses update the phylogeny of the Alphaproteobacteria and show that the parasitic Holosporales is a derived group within the Rhodospirillales order which comprises primarily free-living alphaproteobacteria.

From now on, nanopore metagenomics can be used to monitor aquatic environments with high spatiotemporal resolution, by use of a benchmarked, standardised and cost-effective workflow.