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    1. Neuroscience

    Open-source tools for behavioral video analysis: Setup, methods, and best practices

    Kevin Luxem, Jennifer J Sun ... Mark Laubach
    Methods for video analysis of behaving animals are described, with guidance provided on setting up the methods in a research laboratory and best practices for experimenters and developers.
    1. Evolutionary Biology
    2. Genetics and Genomics

    Broad-scale variation in human genetic diversity levels is predicted by purifying selection on coding and non-coding elements

    David A Murphy, Eyal Elyashiv ... Guy Sella
    Background selection is shown to be the dominant mode of linked selection in humans, with marked effects on diversity levels throughout autosomes.
    1. Cell Biology
    2. Immunology and Inflammation

    Deep-learning-based three-dimensional label-free tracking and analysis of immunological synapses of CAR-T cells

    Moosung Lee, Young-Ho Lee ... YongKeun Park
    Rapid, label-free, volumetric, and automated assessment of the immunological synapse dynamics is demonstrated by combining optical diffraction tomography and deep-learning-based segmentation, providing a new option for immunological research.
    1. Neuroscience

    Dopaminergic modulation of the exploration/exploitation trade-off in human decision-making

    Karima Chakroun, David Mathar ... Jan Peters
    Dopamine attenuates exploration during decision-making via a reduction of neural tracking of uncertainty.
    1. Neuroscience

    Neurogenetic dissection of the Drosophila lateral horn reveals major outputs, diverse behavioural functions, and interactions with the mushroom body

    Michael-John Dolan, Shahar Frechter ... Gregory SXE Jefferis
    The generation and systematic characterisation of driver lines labelling a large number of neurons in the Drosophila innate olfactory processing centre bridges electron microscopy neuronal reconstructions, circuits and behaviour.
    1. Computational and Systems Biology
    2. Immunology and Inflammation

    The naive T-cell receptor repertoire has an extremely broad distribution of clone sizes

    Peter C de Greef, Theres Oakes ... Rob J de Boer
    Combining experiments, bioinformatics, and mathematical modelling, we find large heterogeneity in naive T cell clone sizes.
    1. Ecology

    Mass enhances speed but diminishes turn capacity in terrestrial pursuit predators

    Rory P Wilson, Iwan W Griffiths ... David M Scantlebury
    Increased mass enhances speed but compromises turning capacity in pursuit predators; this has widespread ramifications for the best strategies for predators and prey during chases according to their relative masses.
    1. Neuroscience

    Distinct lateral inhibitory circuits drive parallel processing of sensory information in the mammalian olfactory bulb

    Matthew A Geramita, Shawn D Burton, Nathan N Urban
    Distinct lateral inhibitory circuits affect spiking in olfactory bulb mitral and tufted cells differently, which ultimately allows each cell type to best discriminate between similar odors in separate concentration ranges.
    1. Computational and Systems Biology
    2. Epidemiology and Global Health

    Inference and control of the nosocomial transmission of methicillin-resistant Staphylococcus aureus

    Sen Pei, Flaviano Morone ... Jeffrey L Shaman
    The asymptomatic colonization and importation of methicillin-resistant Staphylococcus aureus (MRSA) in hospital settings can be inferred from observed cases using combined model-inference methods and used to inform improved interventions.
  1. Scientific Publishing: Beyond scoops to best practices

    Eve Marder
    Authors submitting a manuscript to eLife are encouraged to upload it to a recognized preprint server at the same time in order to make their results available as quickly and as widely as possible.