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    1. Microbiology and Infectious Disease

    Tools and methods for high-throughput single-cell imaging with the mother machine

    Ryan Thiermann, Michael Sandler ... Suckjoon Jun
    A user-friendly pipeline for analysis of time-lapse imaging data from the microfluidic mother machine.
    1. Structural Biology and Molecular Biophysics

    Hexameric helicase G40P unwinds DNA in single base pair steps

    Michael Schlierf, Ganggang Wang ... Taekjip Ha
    Single-molecule FRET experiments reveal that G40P, a DnaB-like helicase, unwinds double-stranded DNA in single base pair steps and its processivity is enhanced by host primase DnaG.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    CarD uses a minor groove wedge mechanism to stabilize the RNA polymerase open promoter complex

    Brian Bae, James Chen ... Elizabeth A Campbell
    The crystal structure of Thermus transcription activation complexes containing the transcriptional activator CarD reveals a new mechanism for the activation of transcription.
    1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    Pan-genome analysis identifies intersecting roles for Pseudomonas specialized metabolites in potato pathogen inhibition

    Alba Pacheco-Moreno, Francesca L Stefanato ... Andrew W Truman
    Genomics and genetics were used to identify specialized metabolites employed by Pseudomonas bacteria to suppress the growth of potato pathogens, which revealed a key role for cyclic lipopeptides.
    1. Developmental Biology

    Hedgehog signaling patterns the oral-aboral axis of the mandibular arch

    Jingyue Xu, Han Liu ... Rulang Jiang
    SHH signaling acts through FOXF1/2 transcription factors and antagonizes BMP signaling to specify oral fate and pattern the oral-aboral axis of the mandibular arch to ensure proper formation of jaws.
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Cross-talk between individual phenol-soluble modulins in Staphylococcus aureus biofilm enables rapid and efficient amyloid formation

    Masihuz Zaman, Maria Andreasen
    Inter-molecular cross-seeding between phenol-soluble modulins facilitates rapid and efficient self-assembly in Staphylococcus aureus biofilms revealing a complex molecular interplay between distinct phenol-soluble modulins orchestrating functional amyloid formation.
    1. Developmental Biology
    2. Stem Cells and Regenerative Medicine

    Cell and molecular transitions during efficient dedifferentiation

    John ME Nichols, Vlatka Antolović ... Jonathan R Chubb
    Efficient dedifferentiation is characterized by robustness to mutation, flexibility in ordering of cellular events and reversal of developmental changes along a single gene expression trajectory.
    1. Genetics and Genomics
    2. Microbiology and Infectious Disease

    A single-cell atlas of the miracidium larva of Schistosoma mansoni reveals cell types, developmental pathways, and tissue architecture

    Teresa Attenborough, Kate A Rawlinson ... Matthew Berriman
    A comprehensive description of gene expression and organisation in the cells that constitute the blood fluke larvae responsible for finding and invading its intermediate host.
    1. Microbiology and Infectious Disease

    WhyD tailors surface polymers to prevent premature bacteriolysis and direct cell elongation in Streptococcus pneumoniae

    Josué Flores-Kim, Genevieve S Dobihal ... David Z Rudner
    Selective removal of Wall Teichoic Acids from the envelope of Streptococcus pneumoniae prevents bacteriolysis and directs zonal cell wall synthesis.
    1. Ecology
    2. Microbiology and Infectious Disease

    Freshwater monitoring by nanopore sequencing

    Lara Urban, Andre Holzer ... Maximilian R Stammnitz
    From now on, nanopore metagenomics can be used to monitor aquatic environments with high spatiotemporal resolution, by use of a benchmarked, standardised and cost-effective workflow.