Viral transduction and gene manipulation of adult human brain slices will be of great value allowing investigations including therapeutic screening, electrophysiological and structural studies of properties of human CNS circuitry.
Harshvardhan Gazula, Henry FJ Tregidgo ... Juan E Iglesias
New open-source software enables, for the first time, extraction of quantitative information from brain dissection photographs that are routinely taken at brain banks for archiving purposes but otherwise not exploited.
Regulation of Cav2.3 Ca2+ channels by membrane-bound β2 subunit splice variants permits long-lasting channel activity even during prolonged and continuous activity in dopamine neurons, with implications for Parkinson's disease pathophysiology.
Teresa WM Fan, Richard M Higashi ... Andrew N Lane
Pembrolizumab activates innate immune metabolism and function in primary human non-small cell lung cancer, whereas Pembrolizumab and beta-glucan synergize in enhancing immune metabolism and tumoridical action in brain-metastasized lung cancer.
The polyQ tract of pathogenic Huntingtin causes aggregation when expanded in Huntington’s disease, but its two flanking domains control its conformational landscape, proteostasis and neurotoxicity.
Electrophysiological study in human brain slices reveals that short-term synaptic plasticity from presynaptic pyramidal neuron to postsynaptic interneuron connections are target-cell specific based on their subclass interneuron identity.
Increases of extracellular potassium silence capillary pericyte calcium signaling, suggesting a participation of capillary pericytes in the potassium-mediated neurovascular communication.
Simon Chamberland, Helen H Yang ... François St-Pierre
The combination of a new genetically encoded voltage indicator and fast two-photon imaging methods enables detection of rapid neural electrical activity in organotypic slice cultures and in living flies.
