A structural and biochemical approach shows that CTP binding and hydrolysis regulate nucleation, spreading, and recycling of a chromosome segregation protein ParB.
Genetic and microscopy analyses identify a programmed cell death mechanism that kills a cell subpopulation in a bacterial biofilm where oxygen is limiting, thereby promoting dispersion of newborn motile cells through the action of DNA released by dead cells.
Human chromosome-microtubule attachments are stabilised by Astrin-mediated dynamic delivery of PP1 phosphatase to the attachment site, which ensures the normal segregation of chromosomes.
Matthieu Bergé, Julian Pezzatti ... Patrick H Viollier
A key enzyme of central energy metabolism, citrate synthase, regulates bacterial cell cycle progression at a very specific stage (S-phase) and independently of its enzymatic activity.
The architecture of the bacterial cytokinetic ring in cells and in artificial liposome reconstitutions has been described using electron microscopy, leading to a mechanism of constriction.
The prokaryotic actin homologue MreB forms antiparallel double filaments in vitro and in vivo, an architecture that is unprecedented among the actin family of proteins.
Single-molecule experiments reveal that plectonemic supercoils occupy specific positions on DNA and a physical model relates this to the intrinsic curvature, providing an insight into how supercoiling organizes the genome.