A multidisciplinary platform featured by patient-derived RPEs is established to study the disease-causing mechanisms of BEST1 mutations, and demonstrates gene-supplemented rescue of the mutation-caused deficiency in Ca2+-dependent Cl- current in human RPE.
Gating currents analysis at different Ca2+ concentrations of BK channels reveals a strong allosteric coupling between Ca2+- and voltage-sensing modules via equivalent contributions exerted by the RCK1 and RCK2 Ca2+-sites.
Changes in Shank gene dosage alter voltage-activated calcium current and calcium-activated gene expression in a manner that parallels the effects of human Shank copy number variation on psychiatric disease risk.
The principal potassium ion channel in human sperm, Slo3, is primarily activated by calcium ions and controls the membrane potential of human sperm by intracellular calcium ion levels rather than intracellular pH.
A functional link between representative family members of the CLCA channel regulator family and TMEM16 channels suggests that these protein families may cooperate in influencing multiple homeostatic and disease physiologies.
ArcLight, a popular optogenetic reporter of voltage, is studied at both single-molecule and macroscopic levels, which leads to new mechanistic understanding and to the rational design of a faster reporter.
In-planta ancestral protein resurrection of the female determinant of self-incompatibility specificity in Arabidopsis halleri demonstrates that two allelic variants currently segregating as distinct receptor-ligand combinations diverged through an asymmetrical process.
Neural populations may depend on balanced recurrent connectivity to produce an efficient stimulus representation while also maintaining an accurate stimulus encoding despite the variability introduced by adapting neural responses.