Linker histone H1.8 shapes mitotic chromosomes by tuning the number and size of condensin-dependent DNA loops and suppressing condensin and DNA topoisomerase II-dependent individualization.
An optimized 3D fluorescence co-localization method is a useful toolkit to obtain cellular 3D separations between green and red labeled protein domains with nanometer-scale accuracy using light microscopy.
Reconstitution and cryo-EM reconstruction of the yeast Ctf19 complex (constitutive centromere associated network, or CCAN, in other organisms) shows how inner kinetochore proteins assemble.
Ines A Drinnenberg, Dakota deYoung ... Harmit Singh Malik
CenH3, the defining component of centromeres in almost all eukaryotes, was independently lost in four insect lineages that transitioned from monocentricity to holocentricity.