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    1. Chromosomes and Gene Expression

    Linker histone H1.8 inhibits chromatin binding of condensins and DNA topoisomerase II to tune chromosome length and individualization

    Pavan Choppakatla, Bastiaan Dekker ... Hironori Funabiki
    Linker histone H1.8 shapes mitotic chromosomes by tuning the number and size of condensin-dependent DNA loops and suppressing condensin and DNA topoisomerase II-dependent individualization.
    1. Structural Biology and Molecular Biophysics

    The structure of the yeast Ctf3 complex

    Stephen M Hinshaw, Andrew N Dates, Stephen C Harrison
    The cryo-EM structure of the yeast Ctf3 complex enables near-atomic modeling of most Ctf19c/CCAN factors and their interactions.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Heterochromatin assembly and transcriptome repression by Set1 in coordination with a class II histone deacetylase

    David R Lorenz, Lauren F Meyer ... Hugh P Cam
    Set1 has roles in chromatin organization and transcriptome control that are largely independent of histone H3 lysine 4 methylation.
    1. Cell Biology

    An optimized method for 3D fluorescence co-localization applied to human kinetochore protein architecture

    Aussie Suzuki, Sarah K Long, Edward D Salmon
    An optimized 3D fluorescence co-localization method is a useful toolkit to obtain cellular 3D separations between green and red labeled protein domains with nanometer-scale accuracy using light microscopy.
    1. Cell Biology
    2. Plant Biology

    Kinetochore protein depletion underlies cytokinesis failure and somatic polyploidization in the moss Physcomitrella patens

    Elena Kozgunova, Momoko Nishina, Gohta Goshima
    Lagging chromosomes in anaphase inhibits cytokinesis in plant cells.
    1. Structural Biology and Molecular Biophysics

    The structure of the Ctf19c/CCAN from budding yeast

    Stephen M Hinshaw, Stephen C Harrison
    Reconstitution and cryo-EM reconstruction of the yeast Ctf19 complex (constitutive centromere associated network, or CCAN, in other organisms) shows how inner kinetochore proteins assemble.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Multivalency of NDC80 in the outer kinetochore is essential to track shortening microtubules and generate forces

    Vladimir A Volkov, Pim J Huis in 't Veld ... Andrea Musacchio
    Multiple NDC80 complexes in the outer kinetochore cooperate to bind the ends of depolymerizing microtubules and harness their power stroke.
    1. Cell Biology

    BUB-1 and CENP-C recruit PLK-1 to control chromosome alignment and segregation during meiosis I in C. elegans oocytes

    Samuel JP Taylor, Laura Bel Borja ... Federico Pelisch
    PLK-1 regulates multiple steps during oocyte meiosis I and is recruited to meiotic chromosomes through a dual mechanism involving BUB-1 and CENP-C.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Recurrent loss of CenH3 is associated with independent transitions to holocentricity in insects

    Ines A Drinnenberg, Dakota deYoung ... Harmit Singh Malik
    CenH3, the defining component of centromeres in almost all eukaryotes, was independently lost in four insect lineages that transitioned from monocentricity to holocentricity.
    1. Chromosomes and Gene Expression
    2. Genetics and Genomics

    Spatial inter-centromeric interactions facilitated the emergence of evolutionary new centromeres

    Krishnendu Guin, Yao Chen ... Kaustuv Sanyal
    Proximity and homology guided inter-chromosomal translocations drive karyotype evolution.