Promoter interactome maps in human embryonic stem cells (ESCs) and ESC-derived early neuroectodermal progenitors link distal enhancers to putative target genes, reveal lineage-specific cis-regulatory architecture and shed light on the logic of gene regulation by multiple enhancers.
While photoreceptor and bipolar cells exhibit very similar cis-regulatory grammars, subtle differences in homeodomain motif enrichment represent a key distinction driving the divergence in their transcriptomes.
Cloning-free 3Cs technology is developed for the generation of sequence-bias-free covalently closed circular synthesized (3Cs) CRISPR/Cas gRNA libraries that can interrogate the coding and noncoding human genome.
The DNA-bridging efficiency of H-NS, a genome organising and transcription regulatory protein, is modulated by changes in environmental conditions of the cell, which drive a structural rearrangement of the protein.
The human leukemia virus HTLV-1 causes abnormal chromatin looping in tens of thousands of infected T cell clones in each host, and abnormal host transcription both flanking the integrated provirus and at distant loci in cis.