Sea3, a presumed subunit of the GATOR2 complex in fission yeast, is required for the GATOR1 function that attenuates TORC1 in parallel with the TSC complex and Gcn2 kinase.
Natural substrates of the central endoplasmic reticulum quality control glycoprotein sensors UDP-glucose:glycoproteinglucosyltransferase (UGGT)1 and UGGT2 were identified using a glycoproteomics approach and the role for their modification was explored.
Analysis of the Arabidopsis V-ATPase reveals convergent evolution of differential targeting as well as redundancy of the endosomal and vacuolar isoforms during vegetative development.
TANGO1 functions as a linactant filament to stabilize shallow COPII-coated buds, and after which membrane tension regulation, possibly mediated by TANGO1-controlled membrane fusion, facilitates bud elongation for procollagen export.
Erythroid-enriched BMP2K kinase, in addition to its predicted function in endocytosis, regulates distribution and abundance of COPII assemblies and autophagic degradation through opposing actions of its two splicing variants.
An unbiased model for the self-organisation of the Golgi apparatus displays either anterograde vesicular transport or cisternal maturation depending on ratios of budding, fusion and biochemical conversion rates.
In yeast, a fluorescent vacuolar cargo departs from Golgi cisternae early in the TGN stage of maturation, and traffic from prevacuolar endosomes to the vacuole apparently involves a kiss-and-run mechanism.
The first human TANGO1-associated syndromal disease manifests as impaired collagen secretion, highlighting the importance of TANGO1 in human pathophysiology.
