Calreticulin, beyond its known role as a chaperone, also serves as an endoplasmic reticulum repressor of ATF6⍺, selectively regulating one arm of the unfolded protein response.
Matthew F Pech, Linda E Fong ... Jeffrey Settleman
CRISPR/Cas9 screens reveal a role for the ubiquitin ligase substrate adaptor DCAF15 in the immune surveillance of cancer cells by natural killer cells.
A new approach combines guide RNA multiplexing with CRISPR activation and interference to facilitate functional studies of transposable elements present in hundreds of copies throughout the human genome.
Comprehensive analysis of telomere maintenance from transcriptomic, epigenetic, and loss-of-function profiles of cancer cell lines elucidates features of telomere regulation in cancer.
The catalytic activation of the Cas9 HNH domain involves a unique conformational change in the ββα-metal motif that is collectively driven by the interactions with the metal ions and DNA.
Genetic studies reveal that mosaic inactivation of ccm2 causes characteristic cerebral cavernous malformations in adult zebrafish and aberrant responses to blood flow that induce the formation of a lethal embryonic multi-cavernous venous malformation.
Saskia MA de Man, Gooitzen Zwanenburg ... Renée van Amerongen
Genome editing, advanced live-cell microscopy, and computational modeling were combined to measure WNT/CTNNB1 signaling parameters at the single molecule level, revealing critical regulatory nodes in this important signal transduction pathway.
