Pooled CRISPR knockout screening in Drosophila cells enables high-resolution, genome-wide functional genomic comparisons in cell-lines across vast evolutionary distance.
Functional genomic screening reveals new synthetic lethality with and modes of resistance to epidermal growth factor receptor (EGFR) targeted therapy in EGFR-mutant human lung cancer.
Integrated modeling of sgRNA positioning, chromatin accessibility, and sequence features enables accurate prediction of effective target sites for CRISPR-mediated transcriptional modulation and design of highly active libraries for genome-scale genetic screens.
The HOXA9 reporter and genetic screens facilitated the functional interrogation of the HOXA9 regulome and advanced our understanding of the molecular regulation network in HOXA9-driven leukemia.
A screen using artificially barcoded, exosomal microRNAs, paired with CRISPR guide RNAs, helped identify new players in multivesicular endosome exocytosis and a role for Wnt signaling.
CRISPR/Cas9 screens reveal a role for the ubiquitin ligase substrate adaptor DCAF15 in the immune surveillance of cancer cells by natural killer cells.
Drugs in a curative chemotherapy regimen are independently effective and resisted by different mechanisms, so cancer cells have little chance of surviving all drugs, and this benefit occurs without synergistic interactions.