Pooled CRISPR knockout screening in Drosophila cells enables high-resolution, genome-wide functional genomic comparisons in cell-lines across vast evolutionary distance.

FACS-based pooled CRISPR screening is a powerful forward genetic tool to interrogate cellular pathways and targets that modulate protein fate.

Functional genomic screening reveals new synthetic lethality with and modes of resistance to epidermal growth factor receptor (EGFR) targeted therapy in EGFR-mutant human lung cancer.

Integrated modeling of sgRNA positioning, chromatin accessibility, and sequence features enables accurate prediction of effective target sites for CRISPR-mediated transcriptional modulation and design of highly active libraries for genome-scale genetic screens.

The HOXA9 reporter and genetic screens facilitated the functional interrogation of the HOXA9 regulome and advanced our understanding of the molecular regulation network in HOXA9-driven leukemia.

Zebrafish knockouts can be generated in a few hours directly from wild-type eggs and are suitable for studying continuous traits, including behaviour.

A screen using artificially barcoded, exosomal microRNAs, paired with CRISPR guide RNAs, helped identify new players in multivesicular endosome exocytosis and a role for Wnt signaling.

A novel CRISPR-based genetic screen of candidate regeneration genes in haploid axolotl limbs reveals two genes required for proper regeneration.

CRISPR/Cas9 screens reveal a role for the ubiquitin ligase substrate adaptor DCAF15 in the immune surveillance of cancer cells by natural killer cells.

Drugs in a curative chemotherapy regimen are independently effective and resisted by different mechanisms, so cancer cells have little chance of surviving all drugs, and this benefit occurs without synergistic interactions.