583 results found
    1. Chromosomes and Gene Expression
    2. Human Biology and Medicine

    Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering

    Kunwoo Lee et al.
    The guide RNA and donor DNA of the CRISPR/Cas system tolerate large chemical modifications and can be engineered for enhanced delivery and gene editing.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    Nucleosome breathing and remodeling constrain CRISPR-Cas9 function

    R Stefan Isaac et al.
    The inhibitory influence of nucleosomes on CRISPR-Cas9 is mitigated by nucleosomal DNA breathing and chromatin remodeling.
    1. Developmental Biology and Stem Cells
    2. Genetics and Genomics

    Multiplexed genetic engineering of human hematopoietic stem and progenitor cells using CRISPR/Cas9 and AAV6

    Rasmus O Bak et al.
    The CRISPR/Cas9 system can be used with recombinant AAV6 donor delivery to facilitate simultaneous, targeted integration into multiple genetic loci in hematopoietic stem and progrenitor cells.
    1. Cell Biology
    2. Chromosomes and Gene Expression

    Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery

    Steven Lin et al.
    Building on previous work (Jinek et al., 2013), we report a simple and robust system to achieve high fidelity and high efficiency (30% of homologous recombination) genome engineering by homology-directed repair pathway in human cells using cell cycle synchronization and timed delivery of Cas9 ribonucleoprotein complexes.
    1. Biochemistry and Chemical Biology

    RNA-dependent RNA targeting by CRISPR-Cas9

    Steven C Strutt et al.
    Divergent Cas9 enzymes direct site-specific single-stranded RNA cleavage, reducing infection by RNA phage in vivo and enabling programmable, PAM-independent repression of gene expression in bacteria.
    1. Microbiology and Infectious Disease

    Exploiting CRISPR-Cas to manipulate Enterococcus faecalis populations

    Karthik Hullahalli et al.
    Conflicts between CRISPR-Cas systems and antibiotic resistance plasmids can be exploited to selectively eliminate antibiotic resistance from Enterococcus faecalis populations.
    1. Developmental Biology and Stem Cells
    2. Genetics and Genomics

    CRISPR/Cas9 and active genetics-based trans-species replacement of the endogenous Drosophila kni-L2 CRM reveals unexpected complexity

    Xiang-Ru Shannon Xu et al.
    Dissection of a cis-regulatory element (CRM) in its native chromosomal context using CRISPR/Cas9 editing and novel 'Active Genetics' reveals new features of CRM function and insights into how such regulatory elements change during evolution.
    1. Microbiology and Infectious Disease

    Type III CRISPR-Cas systems can provide redundancy to counteract viral escape from type I systems

    Sukrit Silas et al.
    Cooperation between evolutionarily disparate CRISPR-Cas modules allows bacteria to counter mutational escape by viruses.
    1. Ecology
    2. Chromosomes and Gene Expression

    Emerging Technology: Concerning RNA-guided gene drives for the alteration of wild populations

    Kevin M Esvelt et al.
    Combining CRISPR/Cas9 genome editing with gene drives may enable scientists to reversibly edit the genomes of diverse wild populations, an advance that could help eliminate diseases, support sustainable agriculture, and control invasive species.

Refine your results by:

Type
Research categories