Integrated modeling of sgRNA positioning, chromatin accessibility, and sequence features enables accurate prediction of effective target sites for CRISPR-mediated transcriptional modulation and design of highly active libraries for genome-scale genetic screens.

Newly developed, highly active CRISPR interference single guide RNA libraries and effectors together enable high-quality genetic screens across cell models.

Taxonomy of seizure dynamics (TSD) provides a rigorous method for classifying and quantifying seizures and a principled framework for understanding seizure initiation and propagation.

Pooled CRISPR knockout screening in Drosophila cells enables high-resolution, genome-wide functional genomic comparisons in cell-lines across vast evolutionary distance.

Cooperation between evolutionarily disparate CRISPR-Cas modules allows bacteria to counter mutational escape by viruses.

Conflicts between CRISPR-Cas systems and antibiotic resistance plasmids can be exploited to selectively eliminate antibiotic resistance from Enterococcus faecalis populations.

During CRISPR adaptation, Cas4 forms a ternary complex with the Cas1-Cas2 spacer integration complex, an interaction that coordinates substrate hand-off following precise, PAM-dependent prespacer processing prior to integration.

The protein p53 negatively impacts the ability of a CRISPR screen to discriminate between essential and non-essential genes, hence, p53 status should be considered in these screens.

A high-throughput functional genomics approach combining inducible CRISPR-interference and quantitative imaging yields an atlas of 'phenoprints' to guide gene function assignments, identify metabolic pathway-specific morphotypes, and inform antibiotic mechanism-of-action studies.

Many bacteria use the Nus factor antitermination complex to prevent premature Rho-dependent transcription termination of their CRISPR arrays.