565 results found
    1. Chromosomes and Gene Expression
    2. Computational and Systems Biology

    Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in Drosophila cells

    Raghuvir Viswanatha et al.
    Pooled CRISPR knockout screening in Drosophila cells enables high-resolution, genome-wide functional genomic comparisons in cell-lines across vast evolutionary distance.
    1. Biochemistry and Chemical Biology

    Control of cyclic oligoadenylate synthesis in a type III CRISPR system

    Christophe Rouillon et al.
    Generation of the anti-viral second messenger cyclic oligoadenylate by type III CRISPR systems is tightly controlled in response to viral RNA load and sequence.
    1. Developmental Biology
    2. Genetics and Genomics

    CRISPR/Cas9 and active genetics-based trans-species replacement of the endogenous Drosophila kni-L2 CRM reveals unexpected complexity

    Xiang-Ru Shannon Xu et al.
    Dissection of a cis-regulatory element (CRM) in its native chromosomal context using CRISPR/Cas9 editing and novel 'Active Genetics' reveals new features of CRM function and insights into how such regulatory elements change during evolution.
    1. Microbiology and Infectious Disease

    Exploiting CRISPR-Cas to manipulate Enterococcus faecalis populations

    Karthik Hullahalli et al.
    Conflicts between CRISPR-Cas systems and antibiotic resistance plasmids can be exploited to selectively eliminate antibiotic resistance from Enterococcus faecalis populations.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    Nucleosome breathing and remodeling constrain CRISPR-Cas9 function

    R Stefan Isaac et al.
    The inhibitory influence of nucleosomes on CRISPR-Cas9 is mitigated by nucleosomal DNA breathing and chromatin remodeling.
    1. Biochemistry and Chemical Biology
    2. Cell Biology

    Functional CRISPR screening identifies the ufmylation pathway as a regulator of SQSTM1/p62

    Rowena DeJesus et al.
    FACS-based pooled CRISPR screening is a powerful forward genetic tool to interrogate cellular pathways and targets that modulate protein fate.
    1. Chromosomes and Gene Expression
    2. Human Biology and Medicine

    Synthetically modified guide RNA and donor DNA are a versatile platform for CRISPR-Cas9 engineering

    Kunwoo Lee et al.
    The guide RNA and donor DNA of the CRISPR/Cas system tolerate large chemical modifications and can be engineered for enhanced delivery and gene editing.
    1. Structural Biology and Molecular Biophysics
    2. Chromosomes and Gene Expression

    Selection of chromosomal DNA libraries using a multiplex CRISPR system

    Owen W Ryan et al.
    An optimized CRISPR-Cas9 system enables multiplexed genome engineering for evolving biomolecules and pathways from chromosomally integrated DNA libraries.
    1. Genetics and Genomics
    2. Evolutionary Biology

    Current CRISPR gene drive systems are likely to be highly invasive in wild populations

    Charleston Noble et al.
    If released in the wild, current CRISPR-based gene drive systems designed to alter populations could spread much farther than intended, despite the evolution of drive resistance.
    1. Computational and Systems Biology
    2. Chromosomes and Gene Expression

    Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation

    Max A Horlbeck et al.
    Integrated modeling of sgRNA positioning, chromatin accessibility, and sequence features enables accurate prediction of effective target sites for CRISPR-mediated transcriptional modulation and design of highly active libraries for genome-scale genetic screens.

Refine your results by:

Type
Research categories