Phosphorylation of tyrosine 1 in the carboxy-terminal domain (CTD) of the largest subunit of RNA Polymerase (RNAP) II functions to stabilize this domain, and facilitates turnover of upstream antisense RNAs.
Selective sweepstakes from pervasive positive selection, rather than demographic changes or random sweepstakes reproduction, is the primary determinant of reproductive skew and genetic diversity in the highly prolific Atlantic cod.
Julene Madariaga-Marcos, Cesar L Pastrana ... Fernando Moreno-Herrero
A single-molecule biophysical approach reveals that the C-terminal domain of ParB blocks ParB network formation by heterodimerization with the full-length protein, which remains bound to the DNA.
Spencer R Katz, Maksim A Yakovlev ... Keith C Cheng
Melanin staining for micro-CT imaging, using a novel application of ionic silver deposition, enables qualitative and quantitative 3D analysis of zebrafish pigmentation and reveals subtle phenotypes missed by light microscopy.
Lysine mono- and di-methylation are two novel post-translational modifications of RNA polymerase II, which are enriched at promoters of active genes, precede lysine acetylation and mark early stages of transcription.
A theoretical framework for the growth of microtubules quantifies the roles of geometry, mechanics, kinetics and randomness and provides a phase diagram for dynamic instability in these self-assembled polymers.
Santosh Kumar Kuncha, Vinitha Lakshmi Venkadasamy ... Rajan Sankaranarayanan
Identification of a two-tier functional redundancy to combat proteostasis imbalance induced due to tRNA expansion and oxidative stress in multicellular animals.
Shamayeeta Ray, Samuel P Berry ... Rachelle Gaudet
Structures of a transition metal transporter spanning the entire Mn2+ transport cycle reveal distinct coordination geometries and dynamic polar networks that enable Mn2+ import, and a Cd2+-bound structure helps explain how Cd2+ behaves differently as a substrate.
A Sarah Walker, Emma Pritchard ... COVID-19 Infection Survey team
Wide variation in cycle threshold (Ct) values from SARS-CoV-2 RT-PCR tests over calendar time offers the potential for their broader use in public testing programmes as an ‘early-warning’ system for shifts in infectious load and hence transmission.
