Metabolic flux analysis, biochemistry assays, and functional confirmation in mice show that the ubiquitination and degradation of ATP-citrate lyase (ACLY) mediated by CUL3-KLHL25 promotes the switch from fatty acid synthesis to fatty acid oxidation for iTreg differentiation.
The poorly characterized BTB-protein SPOPL is required to maintain the function of the late endosomal system and the endocytic adaptor EPS15 is therein targeted by the SPOPL/Cullin-3 ubiquitin ligase complex for degradation.
Epithelial cells coordinate integrin signaling with protrusion via a two-protein module, with one protein defining localization and downstream signaling, the other serving as a phosphorylation switch and providing negative feedback.
Cryo-EM structures of unliganded SLC1A5 and its complex with glutamine in outward-facing state provide insights into the substrate specificity and transport mechanism and will be helpful for developing selective inhibitors.
A comprehensive whole cell proteomic map describing expression time courses of >6,500 viral and cellular proteins during HIV infection identifies Vif-dependent antagonism of key cellular phosphatase PP2A.