The intrinsic disorder of the circadian clock protein Frequency organizes binding partners, facilitates liquid–liquid phase separation, modulates Frequency phosphorylation, and derives from sequence properties conserved with homologous clock components.
Unveiling the high-confidence protein–protein interaction networks of human and fly α-arrestins provides insights into conserved and species-specific functional roles depending on their protein domains and motifs.
Daniel Muñoz-Reyes, Levi J McClelland ... Maria Jose Sanchez-Barrena
Biochemical, biophysical, and structural approach to determine the molecular basis of the calcium-sensitive regulation of the Gα chaperone and guanine exchange factor Ric-8A by the neuronal calcium sensor NCS-1.
Differential agonist-induced phosphorylation of free fatty acid receptor 2 is shown in different tissues of transgenic mice engineered to express variant forms of the human ortholog of the receptor.
Herschel S Dhekne, Francesca Tonelli ... Suzanne R Pfeffer
Activating mutations in the leucine-rich repeat kinase 2 cause Parkinson’s disease, and an unbiased genome-wide screen revealed an unexpected, specific role for Rab12 in activating this kinase directly for Rab GTPase phosphorylation.
Alex W Chan, Malgorzata Broncel ... Sebastian Lourido
Comprehensive characterization of kinase-dependent phosphorylation in the human pathogen Toxoplasma gondii identifies a trafficking adaptor complex required for virulence factor secretion and infection.
Nydia Tejeda-Munoz, Yagmur Azbazdar ... Edward M De Robertis
The archetypal tumor promoter PMA, which promotes cancer without mutating the DNA, increases Wnt signaling by activating macropinocytosis and membrane trafficking in Xenopus embryos and tumor cells.
