A proteomics-based approach identifies novel substrate proteins of the Lon protease in Caulobacter crescentus and reveals a critical role of Lon in regulating flagella assembly and stalk biogenesis during the cell cycle.
The McsB kinase is critical for protein quality control in Gram-positive bacteria, assembling a gated phosphorylation chamber during heat-shock conditions to selectively mark misfolded proteins for degradation.
Physiological differentiation during symbiosis leads to division of labor between smaller and larger cells in an uncultured bacterial tubeworm symbiont population and results in remarkable metabolic diversity and complexity.
The INM protein LAP1B, an activator of Torsin ATPases, is a chromatin-binding factor that erroneously persists on mitotic chromatin if Torsin functionality is compromised, inducing chromosome segregation defects and binucleation.
Crosslinking the AAA+ protease interface does not abolish protein degradation by ClpAP, establishing that rotation of the AAA+ unfoldase with respect to its partner peptidase is not essential for activity.