181 results found
    1. Cell Biology

    Electron cryo-tomography reveals the subcellular architecture of growing axons in human brain organoids

    Patrick C Hoffmann et al.
    Combining cerebral organoid technology with cryo-correlative microscopy reveals the organization of cytoskeleton, membrane compartments, and protein synthesis machinery contributing to the rapid expansion of developing human axons.
    1. Microbiology and Infectious Disease

    Cryo-EM reveals new species-specific proteins and symmetry elements in the Legionella pneumophila Dot/Icm T4SS

    Michael J Sheedlo et al.
    Structural analysis of the type IV secretion system purified from Legionella pneumophila uncovers new complex components and symmetry mismatches.
    1. Microbiology and Infectious Disease

    In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography

    Mohammed Kaplan et al.
    Electron cryo-tomography reveals that bacteria can form structurally-diverse outer membrane extensions with various protein complexes associated with them.
    1. Plant Biology
    2. Structural Biology and Molecular Biophysics

    In situ cryo-ET structure of phycobilisome–photosystem II supercomplex from red alga

    Meijing Li et al.
    The nature of the phycobilisome–photosystem II supercomplex on the native thylakoid determined with cryo-electron tomography at an unprecedented resolution reveals that one phycobilisome interconnects with six photosystem monomers.
    1. Neuroscience

    Spatially patterned excitatory neuron subtypes and projections of the claustrum

    Sarah R Erwin et al.
    The claustrum comprises spatially patterned excitatory neuron subtypes with distinct molecular and projection properties.
    1. Cell Biology
    2. Structural Biology and Molecular Biophysics

    Structural heterogeneity of cellular K5/K14 filaments as revealed by cryo-electron microscopy

    Miriam S Weber et al.
    Keratins assemble into tubular filaments with a central density and exhibit structural heterogeneity, which is reflected in alterations of the helical arrangement.
    1. Neuroscience
    2. Structural Biology and Molecular Biophysics

    Topography and motion of acid-sensing ion channel intracellular domains

    Tyler Couch et al.
    The cytosolic elements of the acid-sensing ion channel move toward the plasma membrane when the channel is exposed to acidic conditions.
    1. Microbiology and Infectious Disease

    In vivo imaging of retrovirus infection reveals a role for Siglec-1/CD169 in multiple routes of transmission

    Kelsey A Haugh et al.
    Retroviruses exploit the lectin, Siglec-1/CD169, expressed on sentinel macrophages to promote infection during multiple routes of transmission.
    1. Cell Biology
    2. Computational and Systems Biology

    Microtubule reorganization during female meiosis in C. elegans

    Ina Lantzsch et al.
    Structural rearrangements of meiotic spindles during the transition from metaphase to anaphase can be controlled by local changes of microtubule dynamics, such as nucleation and/or turnover, and that katanin promotes microtubule turnover by severing microtubules near the chromosomes.
    1. Structural Biology and Molecular Biophysics

    Locating macromolecular assemblies in cells by 2D template matching with cisTEM

    Bronwyn A Lucas et al.
    2D template matching complements 3D template matching of tomograms, providing fast and specific detection of molecules in cryo-EM images of cells, as well as generating 3D reconstructions from detected targets.

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