787 results found
    1. Biophysics and Structural Biology

    Beam-induced motion correction for sub-megadalton cryo-EM particles

    Sjors HW Scheres
    Building on previous work (Bai et al., 2013), we describe an algorithm that allows cryo-EM structure determination to near-atomic resolution for protein complexes as small as 170 kDa.
    Research Advance
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology

    Using the Volta phase plate with defocus for cryo-EM single particle analysis

    Radostin Danev et al
    Building on previous work in cryo-electron microscopy (Entchev et al, 2015), it shown that a combination of the Volta phase plate and a small amount of defocus can simplify the experimental set-up, increase the data acquisition rate and improve resolution.
    Research Advance Updated
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biochemistry
    2. Biophysics and Structural Biology

    Cryo-EM visualization of the ribosome in termination complex with apo-RF3 and RF1

    Jesper Pallesen et al
    Cryo-electron microscopy has been used to provide a structural interpretation of the complete action cycle of release factor 3 during translation termination, which includes a coordinated sequence of interactions with a class-I release factor and the ribosome.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology

    Cryo-EM structure of the Plasmodium falciparum 80S ribosome bound to the anti-protozoan drug emetine

    Wilson Wong et al
    The molecular mechanism behind how emetine inhibits the ribosome of the human malaria parasite, along with structural details of the complex formed, is revealed at high resolution using cryo-electron microscopy.
    Research Article Updated
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology

    De novo modeling of the F420-reducing [NiFe]-hydrogenase from a methanogenic archaeon by cryo-electron microscopy

    Deryck J Mills et al
    The structure of a large hydrogenase complex involved in the production of methane by microbes has been determined with cryo-electron microscopy.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology

    Low cost, high performance processing of single particle cryo-electron microscopy data in the cloud

    Michael A Cianfrocco, Andres E Leschziner
    A freely available software environment on Amazon's cloud computing network makes processing of cryo-EM data accessible and affordable.
    Tools and Resources Updated
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology

    Atomic model of the F420-reducing [NiFe] hydrogenase by electron cryo-microscopy using a direct electron detector

    Matteo Allegretti et al
    The structure of a large enzyme complex involved in methanogenesis was determined at high resolution by electron cryo-microscopy.
    Research Article
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology

    Cryo-EM single particle analysis with the Volta phase plate

    Radostin Danev, Wolfgang Baumeister
    The Volta phase plate enables in-focus single particle analysis at near atomic resolutions, which could expand the capabilities of cryo-EM, especially for small, flexible or heterogeneous macromolecular samples.
    Tools and Resources Updated
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology

    Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2

    Dari Kimanius et al
    Using GPUs for the costly computations in cryo-EM enables structure determination in mere days on a single workstation.
    Tools and Resources Updated
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf
    1. Biophysics and Structural Biology

    Microscopy: Cryo-EM enters a new era

    Werner Kühlbrandt
    Insight
    Available as full, typeset article htmlAvailable as authors' accepted manuscript pdf

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