A stable tetrameric nucleosome occupies the central segment of each ∼120-bp budding yeast centromere in two rotational phases of both reflectional orientations in vivo.

Internally tagged, functional Cse4/CENP-A/CenH3 histone variant is exclusively centromeric and stable through the budding yeast cell cycle after replacement in S phase.

The number of CENP-A molecules at human centromeres helps to explain how this structure is built and epigenetically inherited.

Distributed cohesin rings flexibly tether the axial element structure of meiotic chromosomes to the underlying chromatin to readily accommodate ongoing transcription.

The meiotic DNA recombination landscape is locally influenced by the kinetochore to minimize potentially deleterious pericentromeric crossover recombination.

CUT&RUN (Cleavage Under Targets & Release Using Nuclease) profiles antibody-targeted DNA-binding proteins in situ with high resolution and low background, providing a simple, robust and scalable alternative to chromatin immunoprecipitation.

Ploidy is stably maintained in Saccharomyces cerevisae by a tight coordination between histone levels and cell cycle events.

An assay to assemble kinetochores de novo was developed and used to identify the role of the CENP-T pathway.

Centromeric histones, the foundation for accurate chromosome segregation, have now been re-engineered to allow for analysis of the stoichiometry of required domains.

Reconstitution and cryo-EM reconstruction of the yeast Ctf19 complex (constitutive centromere associated network, or CCAN, in other organisms) shows how inner kinetochore proteins assemble.