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Quantitative comparison of clathrin-mediated endocytosis in budding and fission yeast identified conserved mechanisms and species-specific adaptations with broad implications that extend from yeast to humans.

The cryo-EM structure of the yeast Ctf3 complex enables near-atomic modeling of most Ctf19c/CCAN factors and their interactions.

The chromosomal passenger complex interacts with the inner kinetochore COMA complex through the Ctf19 C-terminus in vitro which is shown to be important for mediating accurate chromosome segregation.

Reconstitution and cryo-EM reconstruction of the yeast Ctf19 complex (constitutive centromere associated network, or CCAN, in other organisms) shows how inner kinetochore proteins assemble.

Centromeric histones, the foundation for accurate chromosome segregation, have now been re-engineered to allow for analysis of the stoichiometry of required domains.

An assay to assemble kinetochores de novo was developed and used to identify the role of the CENP-T pathway.

Ploidy is stably maintained in Saccharomyces cerevisae by a tight coordination between histone levels and cell cycle events.

CUT&RUN (Cleavage Under Targets & Release Using Nuclease) profiles antibody-targeted DNA-binding proteins in situ with high resolution and low background, providing a simple, robust and scalable alternative to chromatin immunoprecipitation.

The meiotic DNA recombination landscape is locally influenced by the kinetochore to minimize potentially deleterious pericentromeric crossover recombination.

Distributed cohesin rings flexibly tether the axial element structure of meiotic chromosomes to the underlying chromatin to readily accommodate ongoing transcription.