In addition to its academic merits, characterizing the genetic determinants of male (in)fertility and identifying clinically actionable genetic variants can lead to improved diagnosis or even treatment of such conditions.
Integration of structural bioinformatics and free-energy simulations reveals how a helicase switches its function from unwinding to rezipping DNA, during which a key metastable conformation is predicted and verified by single-molecule measurements.
A statistical approach for predicting non-active site residues responsible for allostery, cooperativity, or other subtle but functionally important interactions is described and applied to various protein families.
Cryo-EM and chemical cross-linking studies yield an atomic resolution structure of a conserved remodeling machinery in messenger ribonucleoprotein maturation/nuclear export and insights into its coordinated function with a serine–arginine protein.
Cryo-electron microscopy structures show how the clinically used antimicrobial fidaxomicin binds and inhibits Mycobacterium tuberculosis RNA polymerase by acting like a doorstop to jam the enzyme in an open conformation via the general transcription factor RbpA.