The physical interaction network encoded in the multi-domain protein native structure handles the trade-off between the fast, stable folding and the efficient, reliable function.
The Srs2 helicase functions in Synthesis-Dependent Strand Annealing to avoid crossover formation during homologous recombination by disrupting D-loops that are extended by DNA polymerase delta in an ATP-dependent and direction-specific manner.
Quantitative binding and kinetic assays on DNA polymerases η and δ define the mechanism for polymerase exchange during human translesion DNA synthesis across a UV-induced lesion on the lagging strand.
NusG enhances transcription elongation by stabilizing DNA base pairs immediately upstream of the RNA-DNA hybrid but does not measurably affect the nucleotide incorporation and the forward translocation by RNA polymerase.
Libing Yu, Jared T Winkelman ... Richard H Ebright
Variability in bacterial transcription start site selection involves DNA “scrunching” and “anti-scrunching,” which may represent a general mechanism for start site selection in all organisms.
Karl P Hodel, Richard de Borja ... Zachary F Pursell
When mismatch repair is compromised heterozygous loss of Pol ε proofreading is sufficient to drive a subset of the observed clinical characteristics of Pol ε tumors.
Johannes Rudolph, Jyothi Mahadevan ... Karolin Luger
By moving between DNA segments like a child swinging on monkey bars, poly(ADP-ribose) polymerase 1 explores possible DNA damage sites more effectively.
Thomas R Beattie, Nitin Kapadia ... Rodrigo Reyes-Lamothe
DnaB Helicase is the only stable component of the bacterial replisome as the replicative DNA polymerase frequently exchanges in both leading and lagging strands.
