Two related DNA replication initiation proteins contribute to the decision of whether to enter a new round of the cell division cycle or enter into a period of proliferative quiescence.
The crystal structure of the MCM helicase bound to single-stranded DNA reveals a binding motif that is critical for cell viability, helicase activation and DNA replication.
Evolutionary adaptation to a constitutive perturbation of DNA replication reveals that adaptive mutations in three conserved pathways interact to restore faithful chromosome replication and segregation.
The universal eukaryotic DNA replication kinetics is the consequence of simple physicochemical rules resulting from the localisation of potential replication origins at discrete sites and the diffusion of limiting origin firing factors in the nuclear space.
A mathematical model that combines stochasticity and spatial structure describes the dynamics of the viral population during an infection cycle, and fitting the model to RNA and virus abundances over time shows that poliovirus follows a geometric replication mode.
Duplication of Leishmania chromosomes combines S-phase DNA replication initiated at a single internal region with subtelomeric DNA replication detectable outside S-phase, potentially explaining genome plasticity in this important parasite.
Ubiquitylation of the CMG helicase is inhibited throughout the initiation and elongation phases of chromosome replication by the DNA structure of a replication fork.
The TRAIP ubiquitin ligase is required during mitosis to disassemble the replisome at sites of incomplete DNA replication, and activate the mitotic DNA repair pathway, thus preserving genome integrity.