A biophysically principled algorithm can build quantitative models of protein-DNA binding specificity of unprecedented accuracy from a leading type of high-throughput in vitro binding data.
A kinetic model of morphogen interpretation is more suitable than classic threshold or ratchet models to understand how a signal gradient generates different target gene expression patterns.
DNA motifs tuned for low affinity binding of BMP-induced pMad/Medea transcription factors function to restrict gene activation to small subsets of the many Drosophila neurons that exhibit active BMP signaling.
Live-cell single-molecule tracking reveals that hierarchical cooperation within the Polycomb Cbx7 protein between the low-affinity H3K27me3-binding module and the high-affinity DNA-binding cassette targets Polycomb repressive complex 1 to chromatin.