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    1. Chromosomes and Gene Expression

    Genome-wide mapping of sister chromatid exchange events in single yeast cells using Strand-seq

    Clémence Claussin et al.
    Genome-wide analysis of sister chromatid exchange using single-cell sequencing reveals that most spontaneous sister chromatid exchange events are not due to the repair of double-strand DNA breaks in wild-type yeast cells.
    1. Structural Biology and Molecular Biophysics

    Structural basis for DNA 5´-end resection by RecJ

    Kaiying Cheng et al.
    A structural investigation reveals details of the mechanism by which the nuclease RecJ processes DNA ends to repair double strand breaks.
    1. Chromosomes and Gene Expression

    Engineered proteins detect spontaneous DNA breakage in human and bacterial cells

    Chandan Shee et al.
    Fluorescent derivatives of a bacteriophage protein that binds double-stranded ends can trap and label genome-destabilizing double-strand DNA breaks in bacterial and human cells, and illuminate the origins of spontaneous DNA breakage in both.
    1. Chromosomes and Gene Expression
    2. Structural Biology and Molecular Biophysics

    CtIP forms a tetrameric dumbbell-shaped particle which bridges complex DNA end structures for double-strand break repair

    Oliver J Wilkinson et al.
    Structural and biochemical analysis of human CtIP provides new insights into DNA break recognition, binding and bridging during homologous recombination.
    1. Chromosomes and Gene Expression

    Covalent linkage of the DNA repair template to the CRISPR-Cas9 nuclease enhances homology-directed repair

    Natasa Savic et al.
    Linking the DNA repair template to the Cas9 ribonucleoprotein complex enhances homology-directed repair of the induced DNA double strand break.
    1. Genetics and Genomics

    RNA-programmed genome editing in human cells

    Martin Jinek et al.
    A bacterial protein can be programmed with short RNA molecules to stimulate genome editing at specific sites in human cells.
    1. Chromosomes and Gene Expression

    High-resolution mapping of heteroduplex DNA formed during UV-induced and spontaneous mitotic recombination events in yeast

    Yi Yin et al.
    Genome-wide mapping of heteroduplex DNA (a recombination intermediate) formed during mitotic recombination in yeast demonstrates that the "classical" model of double-strand DNA break repair is inadequate to explain several aspects of mitotic recombination.
    1. Biochemistry and Chemical Biology

    CRISPR-Cas12a exploits R-loop asymmetry to form double-strand breaks

    Joshua C Cofsky et al.
    The DNA flanks on either side of an R-loop differ in stability, and CRISPR-Cas12 enzymes form double-strand breaks by targeting the more unstable flank with their single DNase active site.
    1. Microbiology and Infectious Disease

    Mycobacterium tuberculosis exploits host ATM kinase for survival advantage through SecA2 secretome

    Savita Lochab et al.
    Mycobacterium tuberculosis (Mtb), effectors secreted through SecA2 pathway cause double strand breaks (DSBs) in the host DNA, which in turn activates ATM kinase to gain survival advantages, through Akt.
    1. Cell Biology
    2. Genetics and Genomics

    Selections that isolate recombinant mitochondrial genomes in animals

    Hansong Ma, Patrick H O'Farrell
    Recombinant mitochondrial genomes can be selected, traits can be genetically mapped and new genetic combinations can be made.