2,926 results found
    1. Biochemistry and Chemical Biology

    RNA-guided retargeting of Sleeping Beauty transposition in human cells

    Adrian Kovač et al.
    Fusion proteins composed of the Sleeping Beauty transposase and catalytically inactive Cas9 target transposon integration into genomic regions specified by single guide RNAs.
    1. Chromosomes and Gene Expression

    PARP1-dependent recruitment of the FBXL10-RNF68-RNF2 ubiquitin ligase to sites of DNA damage controls H2A.Z loading

    Gergely Rona et al.
    Upon genotoxic stress, the FBXL10-RNF68-RNF2 ubiquitin ligase complex mono-ubiquitylates histone H2A and mediates H2A/H2A.Z exchange to repress transcription and ensures proper high fidelity homologous recombination repair.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    SMC condensin entraps chromosomal DNA by an ATP hydrolysis dependent loading mechanism in Bacillus subtilis

    Larissa Wilhelm et al.
    The Smc–ScpAB complex-a prokaryotic ancestor of cohesin, condensin and Smc5/6-loads onto the bacterial chromosome by employing ATP hydrolysis to capture DNA fibers within its tripartite ring.
    1. Developmental Biology

    TAF7L modulates brown adipose tissue formation

    Haiying Zhou et al.
    TAF7L acts as a molecular switch that regulates the development of mesenchymal stem cells.
    1. Biochemistry and Chemical Biology

    Stepwise assembly of the human replicative polymerase holoenzyme

    Mark Hedglin et al.
    Fluorescence resonance energy transfer has been used to explore the interactions between DNA polymerases, sliding clamps and clamp loaders as DNA is replicated in human cells.
    1. Chromosomes and Gene Expression

    MCM2–7-dependent cohesin loading during S phase promotes sister-chromatid cohesion

    Ge Zheng et al.
    Systematic analyses of DNA replication machinery components in human cells reveal a requirement of MCM-dependent de novo loading or mobilization of cohesin at replication forks in establishing sister-chromatid cohesion.
    1. Cancer Biology
    2. Cell Biology

    Many si/shRNAs can kill cancer cells by targeting multiple survival genes through an off-target mechanism

    William Putzbach et al.
    Loading of CD95 and CD95L-derived sequences into the RNA-induced silencing complex elicits a distinct form of RNAi-mediated cell death of cancer cells that results from the targeting of multiple survival genes.
    1. Cell Biology
    2. Neuroscience

    C9orf72 arginine-rich dipeptide repeat proteins disrupt karyopherin-mediated nuclear import

    Lindsey R Hayes et al.
    Poly-PR and poly-GR interact with importin β, disrupt importin-cargo loading, and inhibit nuclear import in permeabilized cells in a manner that can be rescued by RNA.
    1. Cell Biology
    2. Microbiology and Infectious Disease

    Point of View: Is cell size a spandrel?

    Ariel Amir
    Analysis of experiments on bacteria suggests that the dependence of cell size on growth rate is not an adaptation but a causal consequence of a regulatory mechanism that controls DNA replication.
    1. Computational and Systems Biology

    Point of View: Data science for the scientific life cycle

    Daphne Ezer, Kirstie Whitaker
    Each stage of the scientific life cycle stands to benefit from the introduction of data science techniques.

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