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    1. Structural Biology and Molecular Biophysics

    cisTEM, user-friendly software for single-particle image processing

    Timothy Grant, Alexis Rohou, Nikolaus Grigorieff
    cisTEM enables fast processing of single-particle cryo-EM data on CPU-based workstations and is easily accessible to new users.
    1. Biochemistry and Chemical Biology
    2. Microbiology and Infectious Disease

    Extracellular electron transfer increases fermentation in lactic acid bacteria via a hybrid metabolism

    Sara Tejedor-Sanz, Eric T Stevens ... Maria L Marco
    Lactic acid bacteria engage a hybrid metabolism which blends features of fermentation and respiration when they perform extracellular electron transfer.
    1. Physics of Living Systems

    Diameter dependence of transport through nuclear pore complex mimics studied using optical nanopores

    Nils Klughammer, Anders Barth ... Cees Dekker
    Nuclear pore complex mimics based on solid-state nanopores show significant selectivity below a diameter of 55 nm, which decreases gradually for larger pore diameters.
    1. Structural Biology and Molecular Biophysics

    Beam-induced motion correction for sub-megadalton cryo-EM particles

    Sjors HW Scheres
    Building on previous work (Bai et al., 2013), we describe an algorithm that allows cryo-EM structure determination to near-atomic resolution for protein complexes as small as 170 kDa.
    1. Biochemistry and Chemical Biology

    Inter-membrane association of the Sec and BAM translocons for bacterial outer-membrane biogenesis

    Sara Alvira, Daniel W Watkins ... Ian Collinson
    The protein translocation apparatus of the inner- (Sec) and outer-membrane (BAM) interact to form a trans-periplasmic super-complex capable of long-range, PMF-dependent conformational changes to facilitate efficient outer-membrane protein maturation.
    1. Structural Biology and Molecular Biophysics
    2. Cell Biology

    Single-protein detection in crowded molecular environments in cryo-EM images

    J Peter Rickgauer, Nikolaus Grigorieff, Winfried Denk
    High-resolution template matching makes small, densely embedded proteins detectable.
    1. Structural Biology and Molecular Biophysics
    2. Microbiology and Infectious Disease

    Functional role of the type 1 pilus rod structure in mediating host-pathogen interactions

    Caitlin N Spaulding, Henry Louis Schreiber IV ... Edward H Egelman
    The helical rod structure and dynamic spring-like properties of the type 1 pilus are evolutionarily fine-tuned for functioning in host-pathogen interactions during urinary tract infection and gut colonization.
    1. Computational and Systems Biology
    2. Microbiology and Infectious Disease

    Functional genomics of lipid metabolism in the oleaginous yeast Rhodosporidium toruloides

    Samuel T Coradetti, Dominic Pinel ... Jeffrey M Skerker
    Using barcoded mutagenesis and a high-throughput genetic screen results in the identification of 150 genes that affect lipid accumulation in a non-model yeast system.
    1. Cell Biology

    Clathrin-independent pathways do not contribute significantly to endocytic flux

    Vassilis Bitsikas, Ivan R Corrêa Jr, Benjamin J Nichols
    Chemical labelling of all primary endocytic vesicles allows the contribution of different endocytic mechanisms to the total endocytic uptake in cultured mammalian cells to be quantified.
    1. Microbiology and Infectious Disease

    Exosomes mediate horizontal transmission of viral pathogens from insect vectors to plant phloem

    Qian Chen, Yuyan Liu ... Taiyun Wei
    An important rice reovirus hijacks exosomes to traverse the apical plasmalemma into saliva-stored cavities in the salivary glands of insect vectors, facilitating viral horizontal transmission into rice phloem.