566 results found
    1. Chromosomes and Gene Expression

    CMG helicase disassembly is controlled by replication fork DNA, replisome components and a ubiquitin threshold

    Tom D Deegan et al.
    Ubiquitylation of the CMG helicase is inhibited throughout the initiation and elongation phases of chromosome replication by the DNA structure of a replication fork.
    1. Biochemistry and Chemical Biology

    Action of CMG with strand-specific DNA blocks supports an internal unwinding mode for the eukaryotic replicative helicase

    Lance Langston, Mike O’Donnell
    The CMG complex, the replicative helicase in eukaryotes, uses a different mechanism from bacterial and viral helicases by engaging both strands of parental DNA with substantial force and unwinding the duplex within the central channel of CMG.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    DNA binding polarity, dimerization, and ATPase ring remodeling in the CMG helicase of the eukaryotic replisome

    Alessandro Costa et al.
    The Mcm2-7 motor unwinds DNA using an approach distinct from that of superfamily III helicases, and accesses multiple ring configurations and assembly states during the initiation of DNA replication.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    A conserved MCM single-stranded DNA binding element is essential for replication initiation

    Clifford A Froelich et al.
    The crystal structure of the MCM helicase bound to single-stranded DNA reveals a binding motif that is critical for cell viability, helicase activation and DNA replication.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    Nucleosomes influence multiple steps during replication initiation

    Ishara F Azmi et al.
    Nucleosomal DNAs assembled or modified by different chromatin remodeling enzymes differentially impact the origin licensing and helicase activation steps of replication initiation.
    1. Chromosomes and Gene Expression
    2. Structural Biology and Molecular Biophysics

    Analysis of the PcrA-RNA polymerase complex reveals a helicase interaction motif and a role for PcrA/UvrD helicase in the suppression of R-loops

    Inigo Urrutia-Irazabal et al.
    RNA polymerase recruits the PcrA DNA helicase via a conserved interaction motif in order to remove R-loops and prevent conflicts with replication.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Cooperative base pair melting by helicase and polymerase positioned one nucleotide from each other

    Divya Nandakumar et al.
    A detailed model of the T7 replisome with the precise positions of the helicase and polymerase at the replication fork explains how catalysis by the two enzymes is coordinated.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    Mcm10 promotes rapid isomerization of CMG-DNA for replisome bypass of lagging strand DNA blocks

    Lance D Langston et al.
    Biochemical data demonstrate an unexpected and critical function for the enigmatic Mcm10 protein in helping the eukaryotic CMG helicase/replisome bypass roadblocks on the DNA that may also explain Mcm10 function at origins of replication.
    1. Biochemistry and Chemical Biology
    2. Chromosomes and Gene Expression

    A conserved Mcm4 motif is required for Mcm2-7 double-hexamer formation and origin DNA unwinding

    Kanokwan Champasa et al.
    Analysis of an essential motif in Mcm4 provides insights into replicative helicase double-hexamer formation and the first step requiring this intermediate during replication initiation.
    1. Biochemistry and Chemical Biology
    2. Structural Biology and Molecular Biophysics

    Mechanisms of opening and closing of the bacterial replicative helicase

    Jillian Chase et al.
    Analysis of the Escherichia coli DnaB helicase•bacteriophage λ helicase loader (λP) complex provides insights into helicase opening, delivery to the origin and ssDNA entry, and closing in preparation for translocation.

Refine your results by:

Type
Research categories